Prevention of Hip Dysplasia with Thermal Energy

用热能预防髋关节发育不良

• 批准号: 6871716
• 负责人: MANDI J. LOPEZ
• 金额: $ 2.3万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6871716
• 关键词: articular cartilage; biomarker; biomechanics; chondroitin sulfates; collagen; computed axial tomography; disease /disorder prevention /control; dogs; enzyme linked immunosorbent assay; gene expression; hip; hyperthermia therapy; joint disorder; longitudinal animal study; mucopolysaccharides; nonhuman therapy evaluation; osteoarthritis; polymerase chain reaction; proteoglycan; radiography; radioimmunoassay; radiowave radiation; synovial fluid; viscosity

The fundamental goals of this research proposal are to test the hypotheses that: 1) application of thermal energy at 65 degrees C using radio-frequency energy to the coxofemoral joint capsule of puppies with radiographic evidence of joint laxity will result in normal hip congruity, 2) will prevent the development of the lameness and the cartilagenous and bony changes characteristic of CHD, and 3) will not have detrimental effects on the biomechanical or viscoelastic properties of the joint capsular tissue. These investigations will yield insights into the fundamental mechanisms of thermal modification of musculoskeletal soft tissue. This research program is designed to determine whether thermal modification of coxocofemoral joint capsule of 16-week-old puppies with excessive joint: laxity will prevent CHD. Ultimately, this model will serve to evaluate the potential of thermal energy to reduce excessively lax coxofemoral joints in neonatal children and thereby help prevent DDH. The candidate of this application graduated magna cum laude from Humboldt State University with a B.S. in cellular-molecular biology and a minor in chemistry. She worked as a molecular biologist at Amgen, Thousand Oaks, CA, before undertaking and; completing her D.V.M. at the University of California-Davis. Dr. Lopez was awarded 5 scholarships and 2 research grants while a D.V.M. student. She completed an internship at Kansas State University and a large animal surgery residency/Master's degree at they University of Wisconsin- Madison. She is a board certified large animal surgeon. Dr. Lopez's Master's thesis was entitled "Effects of Radio-frequency Energy on Ovine Stifle Joint Capsule", and she performed much of the early radio- frequency work in the Comparative Orthopaedic Research Laboratory. She was awarded an NRSA in 1998 and has performed the background research and preliminary, studies that form the basis for this proposal. She passed her preliminary examinations "With Honors" in November, 1999, and is scheduled to complete her PhD in June 2001. Her ultimate goal is to join the faculty of an academic institution as a well-trained scientist and productive researcher specializing in integrative, translational research. The training environment will be guided toward mentoring the candidate while teaching valuable research techniques necessary to begin an independent research career. The majority of supervision will be provided by Mark D. Markel, an internationally known and respected investigator in the area of thermal tissue modification, Professor of Surgery, and Professor and Chairman of Medical Sciences. The advisory committee will consist of well-respected and highly experienced individuals within the University. The Comparative Orthopaedic Research Laboratory is a well established laboratory that houses multi- disciplinary research programs. The University of Wisconsin provides an exceptional environment for interaction with experts in a variety of fields, and it is well known for its exceptional research programs, especially in the medical sciences.

本研究计划的基本目标是检验以下假设：1)使用射频能量在65℃下对关节松弛的幼犬的髋股关节囊施加热能，将导致正常的髋关节一致性，2)将防止跛行和冠心病特征的软骨和骨改变的发展，3)不会对关节囊组织的生物力学或粘弹性特性产生不利影响。这些研究将深入了解肌肉骨骼软组织热修饰的基本机制。本研究旨在确定16周龄关节过度松弛的幼犬髋股骨关节囊热改性是否能预防冠心病。最终，该模型将用于评估热能减少新生儿髋股关节过度松弛的潜力，从而有助于预防DDH。本申请的候选人以优异的成绩毕业于洪堡州立大学，获得细胞分子生物学学士学位，辅修化学。她曾担任Amgen， Thousand Oaks， CA的分子生物学家，在从事和；在加州大学戴维斯分校完成她的博士学位。洛佩兹博士在D.V.M.学习期间获得了5项奖学金和2项研究补助金。她在堪萨斯州立大学完成了实习，并在威斯康辛大学麦迪逊分校获得了大型动物外科住院医师/硕士学位。她是一个委员会认证的大型动物外科医生。洛佩兹博士的硕士论文题为“射频能量对绵羊膝关节胶囊的影响”，她在比较骨科研究实验室进行了许多早期的射频工作。她于1998年获得了NRSA，并进行了背景研究和初步研究，为本提案奠定了基础。1999年11月，她以优异成绩通过了初试，并计划于2001年6月完成博士学位。她的最终目标是成为一所学术机构的教师，成为一名训练有素的科学家和富有成效的研究人员，专门从事综合转化研究。培训环境将引导指导候选人，同时教授开始独立研究生涯所需的有价值的研究技术。大部分监督将由Mark D. Markel提供，他是热组织修饰领域的国际知名和受人尊敬的研究者，外科教授，医学科学教授和主席。咨询委员会将由大学内德高望重、经验丰富的人士组成。比较骨科研究实验室是一个建立良好的实验室，拥有多学科的研究项目。威斯康星大学为与各个领域的专家互动提供了一个特殊的环境，并且以其卓越的研究项目而闻名，特别是在医学科学方面。