GENOME ANALYSIS OF STREPTOCOCCUS GORDONII

戈登链球菌的基因组分析

• 批准号: 6226290
• 负责人: STEVEN R. GILL
• 金额: $ 22.23万
• 依托单位: INSTITUTE FOR GENOMIC RESEARCH
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6226290
• 关键词: DNA; Streptococcus; bacteria infection mechanism; bacterial endocarditis; computer assisted sequence analysis; cooperative study; dental plaque; genetic library; genetic mapping; genetic strain; genome; high throughput technology; molecular biology information system; nucleic acid sequence; open reading frames; oral bacteria; periodontium disorder; polymerase chain reaction

DESCRIPTION (Investigator?s Abstract): The goal of this project is to determine the complete genome sequence of Streptococcus gordonii, a pioneering colonizer of dental plaque and significant causative bacterium for infective endocarditis. The formation of dental plaque is regulated by complex interactions between the dental surfaces of the host and the surface structures of S.gordonii and other oral bacteria. The basis of the interacting surface structures is highly specific adherence mechanisms controlled by adhesin-receptor partnerships and extracellular glucans. Infective endocarditis often occurs as a result of oral trauma and is likely dependent upon the production of proteases and additional virulence factors. Genetic recombination and transfer of adhesins, antibiotic resistance determinants and possible virulent factors between the oral streptococci may be responsible for evolution of adhesins/receptor pairs and development of antibiotic resistance in S.gordonii. Finally, S.gordonii has been shown to be an effective antigen-delivery vehicle for vaccine development. Through the identification of genome structure, novel adhesions/receptors and regulatory elements, this project will accelerate experimental work directed towards understanding the dynamics of S. gordonii in dental plaque formation and its role in infective endocarditis. The approach to sequencing Streptococcus gordonii strain Challis (NCTC7868) will be a modified whole genome random sequencing strategy successfully used at TIGR to completely sequence 14 prokaryotic genomes. The project will consist of four phases: 1) construction of random small and medium insert plasmid libraries and a large insert BAC library from S. gordonii strain Challis, 2) sequencing both ends of approximately 23,500 small and medium insert clones, 3) sequencing the ends of a set of minimally overlapping BAC clones to provide a scaffolding structure that will minimize the effort required for gap closure and provide confirmation of the underlying assembled structure, and 4) assembly and annotation of the genome to identify structural features, assign gene and functional roles to open reading frames based upon database similarity searches. The data developed from this study will be deposited in several databases, including the TIGR web site. In addition, all clone sets will be made available to the research community.

描述（研究者摘要）：该项目的目标是确定 先驱殖民者戈登链球菌的完整基因组序列 牙菌斑和感染的重要致病菌 心内膜炎。牙菌斑的形成受复杂的调控 宿主牙齿表面与表面结构之间的相互作用 S.gordonii 和其他口腔细菌。相互作用表面的基础 结构是高度特异性的粘附机制，由 粘附素受体伙伴关系和细胞外葡聚糖。感染性心内膜炎 通常是由于口腔外伤而发生，并且可能取决于 蛋白酶和其他毒力因子的产生。基因重组 和粘附素的转移、抗生素耐药性决定因素和可能的 口腔链球菌之间的毒性因素可能是进化的原因 粘附素/受体对的形成和抗生素耐药性的发展 戈登沙门氏菌。最后，S.gordonii 已被证明是一种有效的 用于疫苗开发的抗原递送载体。 通过识别基因组结构、新型粘附/受体和 监管要素，该项目将加速定向试点工作 了解戈登沙门氏菌在牙菌斑形成中的动态 及其在感染性心内膜炎中的作用。测序方法 戈登链球菌菌株 Challis (NCTC7868) 将是一个改良的整体 TIGR 成功使用基因组随机测序策略，完全 测序14个原核生物基因组。该项目将分为四个阶段：1） 随机小中型插入质粒文库和大型质粒文库的构建 插入来自 S. gordonii 菌株 Challis 的 BAC 文库，2) 对 大约 23,500 个小型和中型插入克隆，3) 对 一组最小重叠的 BAC 克隆，以提供脚手架结构 这将最大限度地减少缩小差距所需的努力并提供确认 底层组装结构的组装和注释，以及4） 基因组来识别结构特征，分配基因和功能角色 基于数据库相似性搜索的开放阅读框架。开发的数据 这项研究的成果将存入多个数据库，包括 TIGR 网络 地点。此外，所有克隆集都将可供研究使用 社区。