Acquisition of a Fluidigm C1 Single-Cell Auto Prep System

收购 Fluidigm C1 单细胞自动制备系统

• 批准号: 8825724
• 负责人: STEVEN R. GILL
• 金额: $ 21.33万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8825724
• 关键词: Area; Bioinformatics; Biology; Cancer Biology; Cancer Center; Cells; Clonal Evolution; Clonality; Consultations; Data Analyses; Dentistry; Faculty; Funding; Future; Gene Expression; Genome; Genomics; Goals; Grant; High-Throughput Nucleotide Sequencing; Laboratories; Libraries; Medicine; Methods; Minor; Mission; Molecular Profiling; Monitor; Population; Preparation; Research; Research Design; Research Personnel; Resolution; Resource Sharing; Resources; Sampling; Scientist; Stem Cell Research; Stem cells; System; Technology; United States National Institutes of Health; Universities; base; cancer type; cost effective; experience; genome sequencing; genome-wide analysis; insight; instrument; instrumentation; interest; medical schools; member; response; transcriptome sequencing

 DESCRIPTION (provided by applicant): Single-cell genome and transcriptome sequencing methods have transformed many areas of biology and medicine. These methods enable scientists to identify biologically relevant differences between single cells in a population at higher resolution than previously possible. The goal of this proposal is to acquire funds for a Fluidigm C1 Single-Cell Auto Prep system that will enable the University of Rochester Genomics Research Center (GRC) to provide single-cell genome resources to our faculty and investigators. The GRC,, located within the James P. Wilmot Cancer Center (JPWCC) at the University of Rochester School of Medicine and Dentistry (SMD), is a shared resource laboratory whose mission is to provide high-throughput genome-level analysis to all University faculty and investigators. High-throughput sequencing resources in the GRC include an Illumina HiSeq2500 that was acquired through a 2013 NIH Shared Instrumentation Grant and an Illumina MiSeq that was purchased with internal University funds. The Fluidigm C1 Single-Cell Auto Prep system represents a significant advance in genome-wide analysis at single-cell resolution. Using the C1 Auto Prep in conjunction with existing GRC high-throughput sequencing resources, investigators on the University Main Campus (UR) and SMD will be able to explore several areas of interest, including: 1) determine gene expression profiles of phenotypically defined subpopulations of cells to give insight into heterogenetic responses, 2) investigate clonality and clonal evolution o various cancer types and 3) identify and monitor expression profiles unique to stem cells and their progeny. The Fluidigm instrument will primarily be used by a group of 5 Major and 5 Minor Users who are members of an existing strong Illumina user base with a diversity of NIH funded projects. Support provided by the GRC for high-throughput sequencing includes initial consultation on study design and rationale, sample preparation, library construction, sequencing and Bioinformatic data analysis. Computational support for the GRC is integrated with the Center for Integrated Research Computing (CIRC). These combined resources and support will allow experienced investigators as well as investigators unfamiliar with single-cell genomics to take immediate advantage of the Fluidigm system. Addition of Fluidigm technology as a cost effective shared resource will enable UR/SMD investigators to be at the forefront of cancer biology and stem cell research, provide a substantial benefit to multiple NIH funded projects and stimulate additional projects among new investigators, enabling them develop future NIH funded projects and compete at the highest scientific level.

 描述（由申请人提供）： 单细胞基因组和转录组测序方法已经改变了生物学和医学的许多领域。这些方法使科学家能够以比以前更高的分辨率确定人群中单个细胞之间的生物学相关差异。该提案的目的是为流体C1单细胞自动准备系统获得资金，该系统将使罗切斯特大学基因组研究中心（GRC）能够为我们的教职员工和研究人员提供单细胞基因组资源。位于罗切斯特大学医学与牙科学院（SMD）的James P. Wilmot癌症中心（JPWCC）内的GRC是一个共享的资源实验室，其使命是向所有大学教职员工和研究人员提供高通量基因组级别的分析。 GRC中的高通量测序资源包括通过2013年NIH共享仪器赠款和Illumina Miseq获得的Illumina HISEQ2500，该资源是由内部大学资金购买的。流体C1单细胞自动准备系统在单细胞分辨率下代表全基因组分析的重大进步。 Using the C1 Auto Prep in conjunction with existing GRC high-throughput sequencing resources, investigators on the University Main Campus (UR) and SMD will be able to explore Several areas of interest, including: 1) determine gene expression profiles of phenotypically defined subpopulations of cells to give insight into heterogeneous responses, 2) investigate clonality and clonal evolution o various cancer types and 3) identify and monitor expression profiles unique to stem cells and their进展。 Fluidigm仪器将主要由5个专业和5个未成年人组成的小组使用，这些用户是现有强大的Illumina用户群的成员，拥有多种NIH资助的项目。 GRC为高通量测序提供的支持包括有关研究设计和基本原理，样本准备，图书馆构建，测序和生物信息学数据分析的初步咨询。 GRC的计算支持与综合研究计算中心（CIRC）集成。这些合并的资源和支持将使经验丰富的研究人员以及不熟悉单细胞基因组学的研究人员可以立即利用流体化系统。额外的流体技术作为具有成本效益的共享资源将使UR/SMD研究人员处于癌症生物学和干细胞研究的最前沿，为多个NIH资助的项目提供了可观的好处，并刺激了新研究人员的其他项目，使他们能够发展未来的NIH资助项目并在最高科学水平上竞争。