CORE--GENE TARGETING AND TRANSGENIC MOUSE

基因靶向和转基因小鼠 (GTTM) 共享资源帮助癌症中心成员使用动物模型构建和管理转基因小鼠品系。它由显微注射专家 Sang-Yong Kim 博士操作，并由 Jacek Skowronski 博士（副研究员）监督。 GTTM 共享资源配备了两个显微注射站和支持设备，可通过将 DNA 直接注射到受精卵中来生成转基因小鼠，并使用胚胎干 (ES) 细胞技术构建嵌合小鼠。 GTTM共享资源提供的服务最初将包括通过原核注射生成转基因动物模型、维持能够有效种系贡献以用于基因靶向实验的ES细胞系库存、生成具有基因改变的ES细胞的嵌合小鼠以及小鼠胚胎冷冻保存服务。需要资金购买建立 ES 细胞培养和胚胎冷冻保存服务所需的设备。 GTTM 共享资源最近成立，是为了满足在研究中使用动物模型的癌症中心成员对这些服务不断增长的需求。我们预测 GTTM 共享资源将成为 CSHL 癌症研究人员的重要资源，进一步促进 CSHL 研究人员与世界各地其他研究机构之间的科学互动。

The Gene Targeting and Transgenic Mouse (GTTM) Share Resource assists Cancer Center members who use animal models in constructing and managing genetically altered mouse strains. It is operated by Dr. Sang-Yong Kim, a microinjection expert, and is supervised by Dr. Jacek Skowronski (Associate Investigator). The GTTM Shared Resource is equipped with two microinjection stations and support equipment that allow for generation of transgenic mice by direct DNA injection into fertilized zygotes and construction of chimeric mice using Embryonic Stem (ES) cell technologies. Service provided by the GTTM Shared Resource will initially include generation of transgenic animal models by pro-nuclear injection, maintenance of ES cell line stocks capable of efficient germline contribution for use in gene targeting experiments, generation of chimeric mice with genetically altered ES cells, and mouse embryo cryopreservation services. Funds are requested for the equipment necessary to establish ES cell culture and embryo cryopreservation services. The GTTM Shared Resource was recently established to meet the rising demand for these services from the Cancer Center members that use animal models that use animal models in their research. We predict that the GTTM Shared Resource will become an important resource for cancer researchers at CSHL, further promoting scientific interactions between CSHL investigators, and at other research institutions around the world.