Interactions Discovery Core

交互发现核心摘要 互动发现核心（IDC）将继续支持以细胞蛋白质为重点的互动项目 复合物介导HIV-1感染的早期事件。IDC开展的项目将涉及多个 中心的成员以及外部调查人员。目的1提出了识别细胞蛋白结合 在正选择下特异性地结合到HIV-1衣壳位点。这些高度合作的实验将是 由蛋白质核心和项目3支持，将提供重组CA和/或可溶性寡聚CA 组装，并与HIV病毒学核心合作进行，这将验证病毒学高 优先打击目标病毒学相关靶标随后将进入PCHPI结构测定 渠道.目的2提出鉴定介导HIV-1整合到基因中的CPSF 6相互作用蛋白; 候选的功能性CPSF 6伙伴将在A中进一步研究。恩格尔曼的实验室。目标3建议， 鉴定DNA修复和复制途径中的新HIV Vpr靶点，特别是负责 Vpr诱导DNA损伤检查点。后一项实验将在合作中进行 与项目6，这将验证命中，从而将继续提供新的Vpr的身份 与细胞蛋白质的复合物，用于PCHPI中的机制和结构研究。总的来说，IDC是 PCHPI正在追求的科学的许多方面完全集成和中央。

Interactions Discovery Core Abstract The Interactions Discovery Core (IDC) will continue to support interactive projects focusing on cellular protein complexes mediating early events in HIV-1 infection. The projects carried out by the IDC will involve multiple components of the Center as well as outside investigators. Aim 1 proposes to identify cellular proteins binding specifically to HIV-1 capsid sites under positive selection. These highly collaborative experiments will be supported by the Protein Core and Project 3, which will provide recombinant CA and/or soluble oligomeric CA assemblies, and performed in collaboration with the HIV Virology Core, which will validate virologically high priority hits. The virologically relevant targets will subsequently enter the PCHPI structure determination pipeline. Aim 2 proposes to identify CPSF6 interacting proteins that mediate HIV-1 integration into genes; the candidate functional CPSF6 partners will be investigated further in A. Engelman's lab. Aim 3 proposes to identify novel HIV Vpr targets in DNA repair and replication pathways, and in particular those responsible for the induction of DNA damage checkpoint by Vpr. The latter experiments will be performed in collaboration with Project 6, which will validate the hits, and thereby will continue to provide the identity of new Vpr complexes with cellular proteins for mechanistic and structural studies in the PCHPI. Thus, overall, the IDC is completely integrated and central to many aspect of science being pursued at PCHPI.