Molecular Epidemiology of non Hodgkin's Lymphoma

非霍奇金淋巴瘤的人群病例对照研究 (NHL)将包括旧金山湾的2000名患者和2000名对照 区域。在研究期间，将使用快速病例发现系统来识别旧金山湾区六个县的所有医院的新病例。 控制将通过随机数字拨号和医疗保健进行识别 财务管理档案，并将按年龄与案件进行频率匹配 还有性爱。采访主题将包括医疗、饮食、锻炼、生活方式、 家庭和性史，并将在研究对象的家中进行。 将采集血液和口腔细胞并处理生物样本 将为实验室提供大量的RNA、DNA、血清和活细胞 分析。这些样本将被用来检测关键DNA的多态性 修复、叶酸代谢途径和免疫反应基因，寻找 新的基因和检测艾滋病毒感染。在初步测试完成后 剩下的可存活的血细胞、血清和遗传物质将被储存起来 一种新病毒或基因线索出现的事件.实验室的结果将被用于 通过问卷调查数据按组织学类型评估NHL的危险因素， 年级、性别、种族/民族和艾滋病毒状况。多元Logistic回归分析 在分层存在的情况下遗传病例对照数据的方法如下 用于数据分析。优势包括：1.非霍奇金淋巴瘤患者数量庞大 在旧金山湾区提供了通过以下方式检查关联的能力 性别、种族/民族和组织学亚型；2.创新实验室 能力(例如，分析多个候选基因座的能力 NHL的多态和新的多态)，专家实验室教职员工和 可用于实验室测试的设施；3.使用以下工具完成膳食评估 已建立的饮食调查问卷；4.大量患者和对照组 为评估以前未研究过的种群亚群提供了可能性 以及与非霍奇金淋巴瘤风险相关的暴露之间的相互作用，-S.大型男性同性恋 人口允许探索与艾滋病毒相关的假设和非霍奇金淋巴瘤的风险； 快速发现病例机制将研究对象的损失降至最低，特别是 高级别非霍奇金淋巴瘤患者；7.基于人群的SEER肿瘤登记提供 完整的案例查明；8.稍后的数据将与NCI合并 研究组织类型和种族/民族群体的壁内淋巴瘤项目 与问卷和一些实验室参数有关。

A population-based case-control study of non-Hodgkin's lymphoma (NHL) will include 2000 patients and 2000 controls in the San Francisco Bay Area. A rapid case-finding system will be used to identify new cases in all hospitals in the six-county San Francisco Bay Area during the study period. Controls will be identified by random digit dial and through Health Care Financing Administration files and will be frequency-matched to cases by age and sex. Interview topics will include medical, diet, exercise, lifestyle, family, and sexual histories and will be conducted in study subjects' homes. Blood and buccal cells will be collected and processing of biological samples will provide large amounts of RNA, DNA, serum and viable cells for laboratory analyses. The specimens will be used to test for polymorphisms in key DNA repair, folate metabolism pathway and immune response genes, to search for novel genes and to test for HIV infection. After initial tests are completed the remaining viable blood cells, serum and genetic material will be banked in the event a new virus or genetic clues emerge. The lab results will be used with the questionnaire data to assess risk factors for NHL by histologic type, grade, sex, race/ethnicity, and HIV status. Multiple logistic regression and methods for genetic case-control data in the presence of stratification will be used for data analysis. Strengths include: 1. the large number of NHL patients in the San Francisco Bay Area provides the ability to examine associations by sex, race/ethnicity and histologic subtype; 2. innovative laboratory capabilities (e.g. ability to analyze many candidate loci for genetic polymorphisms and new polymorphisms for NHL), expert laboratory faculty and facilities available for laboratory tests; 3. complete dietary assessment using an established dietary questionnaire; 4. large number of patients and controls provides the potential to evaluate previously unstudied population subgroups and interactions among exposures related to NHL risk,- S. large male homosexual population permits exploration of HIV-related hypotheses and risks for NHL; 6. rapid case-finding mechanism minimizes loss of study subjects, particularly those with high-grade NHL; 7. population-based SEER tumor registry provides complete case ascertainment; 8. data later will be pooled with the NCI intramural lymphoma project to study histologic types and race/ethnicity groups in relation to questionnaire and some laboratory parameters.