Molecular Epidemiology of non Hodgkin's Lymphoma

非霍奇金淋巴瘤的分子流行病学

• 批准号: 6736928
• 负责人: ELIZABETH A. HOLLY
• 金额: $ 151.23万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6736928
• 关键词: DNA repair; HIV infections; cancer risk; clinical research; cytokine; exercise; folate; gender difference; genetic polymorphism; human subject; immunogenetics; lifestyle; microarray technology; neoplasm /cancer epidemiology; neoplasm /cancer genetics; nonHodgkin' s lymphoma; nutrition aspect of cancer; nutrition related tag; oncogenes; racial /ethnic difference; sex behavior; vitamin metabolism

A population-based case-control study of non-Hodgkin's lymphoma (NHL) will include 2000 patients and 2000 controls in the San Francisco Bay Area. A rapid case-finding system will be used to identify new cases in all hospitals in the six-county San Francisco Bay Area during the study period. Controls will be identified by random digit dial and through Health Care Financing Administration files and will be frequency-matched to cases by age and sex. Interview topics will include medical, diet, exercise, lifestyle, family, and sexual histories and will be conducted in study subjects' homes. Blood and buccal cells will be collected and processing of biological samples will provide large amounts of RNA, DNA, serum and viable cells for laboratory analyses. The specimens will be used to test for polymorphisms in key DNA repair, folate metabolism pathway and immune response genes, to search for novel genes and to test for HIV infection. After initial tests are completed the remaining viable blood cells, serum and genetic material will be banked in the event a new virus or genetic clues emerge. The lab results will be used with the questionnaire data to assess risk factors for NHL by histologic type, grade, sex, race/ethnicity, and HIV status. Multiple logistic regression and methods for genetic case-control data in the presence of stratification will be used for data analysis. Strengths include: 1. the large number of NHL patients in the San Francisco Bay Area provides the ability to examine associations by sex, race/ethnicity and histologic subtype; 2. innovative laboratory capabilities (e.g. ability to analyze many candidate loci for genetic polymorphisms and new polymorphisms for NHL), expert laboratory faculty and facilities available for laboratory tests; 3. complete dietary assessment using an established dietary questionnaire; 4. large number of patients and controls provides the potential to evaluate previously unstudied population subgroups and interactions among exposures related to NHL risk,- S. large male homosexual population permits exploration of HIV-related hypotheses and risks for NHL; 6. rapid case-finding mechanism minimizes loss of study subjects, particularly those with high-grade NHL; 7. population-based SEER tumor registry provides complete case ascertainment; 8. data later will be pooled with the NCI intramural lymphoma project to study histologic types and race/ethnicity groups in relation to questionnaire and some laboratory parameters.

非霍奇金淋巴瘤的病例对照研究 (NHL)将包括在旧金山弗朗西斯科湾的2000名患者和2000名对照 区将使用一个快速病例查找系统， 六个县的医院在研究期间的弗朗西斯科湾区。 将通过随机数字拨号和医疗保健识别质控品 财务管理部门存档，并将按年龄与案件频率匹配 和性别面试主题将包括医疗，饮食，锻炼，生活方式， 家庭和性史，并将在研究受试者家中进行。 将采集血液和口腔细胞并处理生物样本 将为实验室提供大量的RNA、DNA、血清和活细胞 分析。这些标本将用于检测关键DNA的多态性 修复、叶酸代谢途径和免疫应答基因，寻找 新的基因和测试艾滋病毒感染。初步测试完成后 剩余的活血细胞、血清和遗传物质将储存在 一旦出现新病毒或基因线索。实验室的结果将被用于 通过问卷调查数据评估组织学类型NHL的危险因素， 年级、性别、种族/民族和艾滋病毒状况。多元logistic回归分析 在存在分层的情况下，遗传病例对照数据的方法将是 用于数据分析。优势包括：1.大量的NHL患者 在旧金山弗朗西斯科湾区， 性别、种族/民族和组织学亚型; 2.创新实验室 能力（例如，分析许多候选基因座以进行遗传分析的能力） 多态性和NHL的新多态性），专家实验室教师和 实验室测试设施; 3.完整的膳食评估， 建立饮食问卷; 4.大量患者和对照 提供了评价以前未研究的人群亚组的可能性 以及与NHL风险相关的暴露之间的相互作用，- S。大男同性恋 人群允许探索HIV相关假设和NHL风险; 6. 快速的病例发现机制最大限度地减少了研究对象的损失，特别是 高级别NHL患者; 7.基于人群的SEER肿瘤登记研究提供了 完整的案件查明; 8.稍后，数据将与NCI合并 研究组织学类型和种族/民族组的壁内淋巴瘤项目 与问卷调查和一些实验室参数有关。