A P. falciparum MSP1 p42/QS-21 malaria vaccine

恶性疟原虫 MSP1 p42/QS-21 疟疾疫苗

• 批准号: 6337444
• 负责人: Sandra Perreira Chang
• 金额: $ 30.25万
• 依托单位: AGENUS, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-15 至 2004-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6337444
• 关键词: Plasmodium falciparum; bioreactors; cell line; immunomodulators; laboratory mouse; malaria; malaria vaccines; method development; protein purification; protozoal antigen; surface antigens; vaccine development; vector vaccine

DESCRIPTION: (Adapted from Applicant's Abstract) Malaria is a disease which affects more than 300 million people each year and is fatal for 1.5 million. The overall objective of this STTR Phase I project is to produce a cGMP malaria vaccine consisting of the 42 kDa C-terminal polypeptide of P. falciparum merozoite surface protein-1 (MSP1.42) to be formulated with QS21 adjuvant for preclinical studies and a future clinical Phase I trial. Evidence is presented that MSP1.42/QS-21 protects against P. falciparum malaria in the Aotus monkey model. Data is also presented supporting the feasibility of large-scale MSP1.42 production in the baculovirus system and its purification by standard chromatography. The goals of the project are (1) production of master cell banks of insect cell lines for virus stock and polypeptide production, respectively; (2) preparation of a virus stock of recombinant MSP1.42 baculovirus; (3) production of bulk MSP1.42 antigen in a bioreactor; (4) purification of MSP1.42; (5) establishment of analytical methods to define product specifications; and (6) evaluation of the immunogenicity of the cGMP MSP1.42/QS21 formulation in mice and Aotus and of its efficacy in Aotus. These studies will provide cGMP material and preclinical information for a clinical trial of MSP1.42/QS21 during STTR Phase II. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述：（改编自申请人摘要）疟疾是一种疾病