Chromatin remodelling and its conserved role in plant immunity

染色质重塑及其在植物免疫中的保守作用

• 批准号: 1642852
• 金额: --
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1642852
• 关键词: Chromatin; remodelling; its; conserved; role

A major common part of plant immune responses is the reprogramming of gene expression. Activation of receptors significantly alters the expression of the plant genome, enabling cellular defence responses to be activated. However, activation of immunity comes with a severe developmental trade-off assumed to be necessary in order to prioritize defence over growth-related cellular functions.Chromatin remodelling is a major regulator of transcription that plays a crucial role in plant growth, and immunity. We have identified a Chromatin-Remodelling Enzyme (CRE1) altering plant homeostasis in a manner that simultaneously increases immunity and growth - pathways that typically act antagonistically. The Arabidopsis mutant of this enzyme (cre1) has enhanced leaf area, increased root length and is more resistant to pathogens. Most importantly, under stress condition the cre1 has bigger leaf area and produces more seeds than the wild type plants. We are interested to exploit the high level of conservation of these enzymes across plant lineages in an effort to engineer high performing tomato and Brassica plants with increased resistance to pathogens, yet minimal impact upon growth. Two main lines of research are considered; gene editing of CRE1 using CRISPR technology and developing chemical inhibitors of the enzyme for foliar applications.

植物免疫反应的一个主要共同部分是基因表达的重编程。受体的激活会显著改变植物基因组的表达，从而激活细胞防御反应。然而，免疫的激活伴随着严重的发育权衡，假设是必要的，以优先考虑防御生长相关的细胞功能。染色质重塑是转录的主要调节因子，在植物生长和免疫中起着至关重要的作用。我们已经鉴定了一种染色质重塑酶（CRE1），其以同时增加免疫力和生长途径的方式改变植物体内平衡，所述免疫力和生长途径通常起拮抗作用。这种酶的拟南芥突变体（cre1）具有增强的叶面积，增加的根长度和对病原体的抗性。最重要的是，在胁迫条件下，cre1比野生型植物具有更大的叶面积和产生更多的种子。我们感兴趣的是利用这些酶在植物谱系中的高水平保守性，以努力工程化高性能的番茄和芸苔属植物，其对病原体的抗性增加，但对生长的影响最小。研究主要有两条主线：使用CRISPR技术对CRE1进行基因编辑，以及开发用于叶面应用的酶的化学抑制剂。