Mechanisms of Spectral Tuning

描述：本项目的重点将是阐明脑出血的机制。 短波视觉色素亚群的光谱调谐。首先， 11-顺式视网膜发色团中席夫碱氮的质子化状态 紫外线、人蓝和牛蓝颜料的含量将由 15N-固态魔角旋转(MAS)核磁共振波谱。第二种是氨基 负责短波组内光谱调谐的酸残基将 被指认出来。最后讨论了短波的光谱调谐机制。 亚组将与视觉色素的其他亚组进行比较。在……里面 特别是斑马鱼蓝色素，因为它 是中波长色素亚群的成员(基于其氨基 酸序列)，但最大值集中在短波长范围内 (419 Nm)。这项工作的结果将有助于阐明光谱的机制。 在视觉颜料之间进行调整，以阐明 人类色觉的进化发展。

DESCRIPTION: The focus of this project will be to elucidate the mechanism of spectral tuning in the subgroup of short-wave visual pigments. First, the protonation state of the Schiff base nitrogen in the 11-cis retinal chromophore of the ultraviolet, human blue and bovine blue pigments will be determined by 15N-solid state magic angle spinning (MAS) NMR spectroscopy. Second the amino acid residues responsible for spectral tuning within the short-wave group will be identified. Finally the mechanism of spectral tuning in the short-wave subgroup will be compared to that of other subgroups of visual pigments. In particular, attention will be focused on the zebrafish blue pigment because it is a member of the middle-wavelength subgroup of pigments (based on its amino acid sequence) but has a maximum centered in the short wavelength range (419nm). Results from this work will shed light on the mechanisms of spectral tuning among the visual pigments to illuminate the mechanistic basis for the evolutionary development of human color vision.