Dysferlin and the emergency vesicle fusion of membrane repair

Dysferlin 和膜修复的紧急囊泡融合

• 批准号: nhmrc : 1103618
• 负责人: A/Pr Sandra Cooper
• 金额: $ 33.1万
• 依托单位: Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: Project Grants
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/dysferlin-emergency-vesicle-membrane-repair/662684
• 关键词: Dysferlin; emergency; vesicle; fusion; membrane

Membrane repair is a vital cell survival mechanism of all eukaryotic cells, using calcium-triggered vesicle fusion to ‘patch’ membrane ruptures. The muscular dystrophy protein dysferlin is a key mediator of membrane repair, although, exactly how dysferlin mends membranes has been unclear. We show that the calcium that floods through membrane tears, activates a group of enzymes called calpains, that specifically cleave dysferlin to release a specialist vesicle-fusion module for membrane repair.

膜修复是所有真核细胞的重要细胞生存机制，利用钙触发的囊泡融合来“修补”膜破裂。肌营养不良蛋白异铁蛋白是膜修复的关键介质，尽管，异铁蛋白究竟如何修复膜还不清楚。我们的研究表明，钙通过膜撕裂，激活一组叫做钙蛋白酶的酶，这种酶能特异性地切割异质蛋白，释放出一种专门的囊泡融合模块，用于膜修复。