Co-evolution of virulence and recognition in rice blast disease

稻瘟病毒力与识别的协同进化

• 批准号: 1654182
• 金额: --
• 依托单位: University of East Anglia
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1654182
• 关键词: Co; evolution; virulence; recognition; rice

Plant diseases are a continuous threat to food production and a major constraint on achieving food security. Receptors of the plant immune system are critical agents in the fight against infection. One set of plant immunoreceptors detect the presence of host-translocated pathogen effector proteins and trigger defence responses. One approach to try and solve the problem of plant diseases is to understand the molecular basis of interactions between plant immunoreceptors and the effectors they have evolved to detect. This allows engineering of these interactions to improve disease resistance. Further, understanding how effectors function in the plant cell, how they promote pathogen growth and disease progression, is fundamentally interesting but also offers opportunity to interfere with disease processes.This project will focus on a family of effector proteins from the most devastating pathogen of rice, the fungus Magnaporthe oryzae. The student will apply biochemical, biophysical, structural and in planta approaches to probe direct physical interactions ("handshakes") between different proteins that help promote disease and trigger resistance respectively. They will test the effects of varying the interfaces of these proteins in vitro and, working with collaborators in Japan, can have their predictions from in vitro experiments tested in vivo.

植物病害是对粮食生产的持续威胁，也是实现粮食安全的主要制约因素。植物免疫系统的受体是抵抗感染的关键因子。一组植物免疫受体检测宿主易位的病原体效应蛋白的存在并触发防御反应。试图解决植物疾病问题的一种方法是了解植物免疫受体和它们进化来检测的效应物之间相互作用的分子基础。这允许工程化这些相互作用以提高抗病性。此外，了解效应子如何在植物细胞中发挥作用，它们如何促进病原体生长和疾病进展，从根本上讲是有趣的，但也提供了干扰疾病过程的机会。本项目将重点关注来自水稻最具破坏性的病原体--真菌Magnaporthe pseudosis的效应子蛋白家族。学生将应用生物化学，生物物理，结构和植物方法来探测不同蛋白质之间的直接物理相互作用（“握手”），分别有助于促进疾病和触发抗性。他们将在体外测试这些蛋白质不同界面的影响，并与日本的合作者合作，可以在体内测试体外实验的预测。

项目成果

The blast pathogen effector AVR-Pik binds and stabilizes rice heavy metal-associated (HMA) proteins to co-opt their function in immunity

• DOI: 10.1101/2020.12.01.406389
• 发表时间: 2020-12
• 期刊: bioRxiv
• 作者: Kaori Oikawa;K. Fujisaki;Motoki Shimizu;Takumi Takeda;Keiichiro Nemoto;H. Saitoh;Akiko Hirabuchi;Yukie Hiraka;Naomi Miyaji;A. Białas;Thorsten Langner;Ronny Kellner;T. Bozkurt;Stella Cesari;T. Kroj;M. Banfield;S. Kamoun;R. Terauchi

Multiple variants of the fungal effector AVR-Pik bind the HMA domain of the rice protein OsHIPP19, providing a foundation to engineer plant defense.

• DOI: 10.1016/j.jbc.2021.100371
• 发表时间: 2021-01
• 期刊: The Journal of biological chemistry
• 作者: Maidment JHR;Franceschetti M;Maqbool A;Saitoh H;Jantasuriyarat C;Kamoun S;Terauchi R;Banfield MJ
• 通讯作者: Banfield MJ

Using a rice blast effector target to engineer NLR immune receptors with novel recognition specificities

使用稻瘟病效应靶标来设计具有新颖识别特异性的 NLR 免疫受体

• 发表时间: 2020
• 作者: Maidment JHR

Multiple variants of the blast fungus effector AVR-Pik bind the HMA domain of the rice protein OsHIPP19 with high affinity

• DOI: 10.1101/2020.12.01.403451
• 发表时间: 2020-12
• 期刊: bioRxiv
• 作者: J. Maidment;M. Franceschetti;A. Maqbool;H. Saitoh;C. Jantasuriyarat;S. Kamoun;R. Terauchi;M. Banfield

Taking the stage: effectors in the spotlight.

• DOI: 10.1016/j.pbi.2017.04.013
• 发表时间: 2017-08
• 期刊: Current opinion in plant biology
• 影响因子: 9.5
• 作者: F. Varden;Juan Carlos De la Concepcion;J. Maidment;M. Banfield