REGULATION OF THE P GINGIVALIS FIMA GENE

本K08申请计划研究细菌菌毛的调节。 革兰氏阴性口腔病原菌，牙龈假单胞菌是。这段假定的时期 对病原菌已经进行了广泛的研究，并有大量的 毒力因子已经确定。然而，即使有的话，也很少 已经被观察到在体内发挥作用。牙龈假单胞菌菌毛 IS被认为是一个结构性毒力因素，因为 这些结构导致细菌对细菌的粘附性很差，甚至没有粘附性 宿主细胞。因为细菌附着在宿主细胞上的能力 寄主相关细菌毒力的关键第一步，即丧失 这一功能对人的生存或健康至关重要。 主持人。亚基蛋白fimbrins的结构基因 它形成了菌毛结构，已经被克隆和测序 从九种牙龈假单胞菌中分离出菌株。除了基因研究， 这种细菌的菌毛已经得到了广泛的研究。 从免疫学、化学和结构上看。

This K08 application plans to study the regulation of fimbriae in the gram negative oral pathogen, P. gingival is. This putative period onto pathogen has been studied extensively and a large number of virulence factors have been determined. However, few if any of them have been observed to function in vivo. The fimbriae of P. gingival is are considered to be a structural virulence factor since loss of these structures results in poor to no adherence the bacterium to host cells. Since the ability of a bacterium to adhere to host cells is an essential first step in host-associated bacterial virulence, loss of this function is critical to survival or the health and well being of the host. The structural gene for fimbrillin, the subunit protein which forms the fimbriae structure, has been cloned and sequenced from nine P. gingival is strains. In addition to the genetic studies, the fimbriae from this bacterium have been extensively studied immunologically, chemically and structurally.