Investigation of a regulatory iron-sulfur complex in a bacterial sigma factor

细菌西格玛因子中调节性铁硫复合物的研究

• 批准号: 1723623
• 金额: --
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1723623
• 关键词: Investigation; regulatory; iron; sulfur; complex

The bacterium Burkholderia thailandensis produces malleobactin which it uses to capture iron. The genes encoding the biosynthesis of malleobactin are under the control of the sigma factor MbaS and are expressed under iron limiting conditions. The characteristics of MbaS suggest that it possesses an iron-sensing domain at its C-terminus. We hypothesise that under iron sufficient conditions, MbaS acquires an iron-sulfur cluster which inhibits association of MbaS-RNA polymerase with promoter DNA.

细菌Burkholderia thailandensis产生用于捕获铁的malleobactin。编码锤骨菌素生物合成的基因受σ因子MbaS的控制，并在铁限制条件下表达。MbaS的特征表明其C-末端具有铁敏感结构域。我们假设，在铁充足的条件下，MbaS获得的铁硫簇，抑制协会的MbaS-RNA聚合酶与启动子DNA。