Polymersomes - can these unique nanoparticles be used to protect the heart?

聚合物囊泡——这些独特的纳米颗粒可以用来保护心脏吗？

• 批准号: 1763912
• 金额: --
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1763912
• 关键词: Polymersomes; these; unique; nanoparticles; used

TA heart attack is the acute event that causes death and injury in patients with coronary artery disease. Currently, restoration of normal blood flow is the main treatment but, paradoxically , this restoration, also causes injury to heart muscle in what is termed "lethal reperfusion injury" (myocardial cell death). Therefore, finding ways to reduce lethal reperfusion injury is vital for these patients.We have recently shown that exosomes - endogenous nanoparticles in the blood - are cardioprotective. However, they are heterogeneous, difficult to purify, and therefore not ideal potential therapeutic agents. Polymersomes, on the other hand, are a totally unique type of nanoparticle we developed that is completely synthetic, can be easily produced, and can be modified as desired to optimize performance. For example, by modifying their surface, they can be designed to home to targets on the surface of specific cell types.Our two groups are collaborating to use polymersomes for the first time to treating lethal reperfusion injury in the heart.The aims of this project are:1. starting with fluorescently labelled polymersomes, to demonstrate their uptake and delivery into the heart, and specifically into cardiac myocytes,2. to load polymersomes with cardioprotective agents and show they can protect the heart against ischaemic and reperfusion injury,3. to modify polymersomes in various ways to improve them, for example targeting them specifically to the injured heart (eg: via LRP1)The primary lab has all cardiovascular protocols in place: human muscle, rodent heart and other cellular models. The secondary lab has everything required for chemical synthesis of polymersomes. We are eager to find an enthusiastic and capable student willing to take this project forward.

TA心脏病发作是导致冠心病患者死亡和受伤的急性事件。目前，恢复正常血流是主要的治疗方法，但矛盾的是，这种恢复也会造成心肌损伤，即所谓的“致命性再灌注损伤”(心肌细胞死亡)。因此，找到减少致命性再灌注损伤的方法对这些患者至关重要。我们最近表明，外切体-血液中的内源性纳米颗粒-具有心脏保护作用。然而，它们是多相的，很难纯化，因此不是理想的潜在治疗药物。另一方面，聚合体是我们开发的一种完全独特的纳米颗粒，它完全是人工合成的，可以很容易地生产，并且可以根据需要进行修改以优化性能。例如，通过修饰它们的表面，它们可以被设计成定位于特定细胞类型表面的靶点。我们两个小组首次合作使用聚合体来治疗心脏的致命再灌注损伤。这个项目的目的是：1.从荧光标记的聚合体开始，证明它们被摄取和输送到心脏，特别是进入心肌细胞，2.用心脏保护剂负载聚合体，并证明它们可以保护心脏免受缺血和再灌注损伤，3.以各种方式修饰聚合体以改善它们，例如，将它们专门定位于受伤的心脏(例如：通过LRP1)，主要实验室已经准备好了所有的心血管方案：人类肌肉、啮齿动物心脏和其他细胞模型。二级实验室拥有化学合成聚合体所需的一切。我们渴望找到一个热情和有能力的学生愿意推进这个项目。