Coiled-coil proteins as nano-wire scaffolds for in vitro and in vivo synthetic biology applications

卷曲螺旋蛋白作为纳米线支架用于体外和体内合成生物学应用

• 批准号: 1771922
• 金额: --
• 依托单位: University of East Anglia
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1771922
• 关键词: Coiled; coil; proteins; nano; wire

One of the exciting areas of synthetic biology capitalises on our knowledge of natural biological material for engineering products with novel functions. In addition to fundamental research, the global synthetic biology market is expected to grow to $38.7 billion by 2020 and the UK Government has identified synthetic biology as one of the 'Eight Great Technologies' in which Britain can play a major role.This work will focus on synthetic biology using coiled-coil oligopeptides in order to generate nanostructures that are used as scaffolds, for example, for tissue regeneration. The building blocks of such nano-structures are based on coiled-coil peptide motifs that exist as part of naturally existing coiled-coil proteins. Our study will investigate the assemblies that are derived from coiled-coil building blocks with unusual characteristics. We have recently shown that the tip organising centre for bacterial polar growth contains a multi-protein assembly of coiled-coil proteins with unusual characteristics. During the proposed project we will derive short coiled-coil oligopeptides based on sequence analysis of naturally occurring coiled-coil proteins. We will use biochemical and biophysical technologies to investigate and characterise the higher order assemblies generated by these oligopeptides. We will also test the effect of mutations on the filamentation or branching characteristics of the nano-filaments formed. These investigations will provide a platform for rational peptide-design for the construction of nano-structures that can act as scaffolds for biological and medical applications.

合成生物学的一个令人兴奋的领域是利用我们对天然生物材料的知识来制造具有新功能的工程产品。除了基础研究，到2020年，全球合成生物学市场预计将增长到387亿美元，英国政府已将合成生物学确定为英国可以发挥主要作用的八大技术之一。这项工作将专注于合成生物学，使用盘绕寡肽来生成纳米结构，用作组织再生的支架。这种纳米结构的基础是作为自然存在的盘绕蛋白的一部分存在的盘绕多肽基序。我们的研究将调查从具有不寻常特性的盘绕线圈积木派生出来的组件。我们最近已经证明，细菌极地生长的末端组织中心包含一个具有不寻常特征的卷曲蛋白质的多蛋白质组装。在拟议的项目中，我们将基于对自然产生的卷曲蛋白的序列分析来获得短卷曲寡肽。我们将使用生化和生物物理技术来研究和表征这些寡肽产生的高阶组装。我们还将测试突变对形成的纳米细丝的丝化或分支特性的影响。这些研究将为构建可作为生物和医学应用支架的纳米结构提供合理的多肽设计平台。