FACS VANTAGE SE CELL SORTER/FLOW CYTOMETER

这是PAR-99- 031项下的共享仪器补助金申请，寻求295，649美元的资金，用于购买最先进的细胞分选机/流式细胞仪，以取代14岁和报废的流式细胞仪。该仪器将放置在位于田纳西大学诺克斯维尔兽医学院的设备齐全的核心设施中。需要该仪器来支持田纳西大学诺克斯维尔兽医学院的3名NIH资助的研究人员（Rouse、Schuller和Wang博士）和来自田纳西大学医学中心诺克斯维尔的1名NIH资助的合作者（Wimalasena博士）的研究。这些调查员目前正在进行的和有待发放的赠款在很大程度上取决于是否有这种工具。所附的Becton Dickinson报价单是针对配备氩紫外激光器、氦氖激光器、脉冲处理器、荧光检测器、第三激光器底座和INDO- 1滤光片组的FACS Vantage TM/SE细胞分选仪。如果获奖，其他供应商将有机会对Becton Dickinson出价。描述了长期财务计划设施总监、技术设施经理和咨询委员会。

This is an application for a shared instrumentation grant under PAR-99- 031 seeking funds in the amount of $295,649 for the purchase of a state of the art cell sorter/flow cytometer to replace a 14 year old and defunct flow cytometer. This instrument will be housed in a fully equipped core facility located in the College of Veterinary Medicine, University of Tennessee Knoxville. This instrument is needed to support the research of 3 NIH funded investigators at the College of Veterinary Medicine, University of Tennessee Knoxville (Drs. Rouse, Schuller, and Wang) and 1 NIH funded collaborator from the Medical Center University of Tennessee, Knoxville (Dr. Wimalasena). The currently active and pending grants of these investigators depend heavily on the availability of this instrument. The attached quotation from Becton Dickinson is for a FACS Vantage TM/SE Cell Sorter with Argon UV laser, helium-neon laser, pulse processor, fluorescence detection, third laser mount, and INDO- 1 filter- set. If an award is made, other vendors will be given the opportunity to bid against Becton Dickinson. A long-term financial plan facility director, technical facility manager, and advisory committee are described.