ZEISS LSM 510 CONFOCAL MICROSCOPE SYSTEM

蔡司 LSM 510 共焦显微镜系统

• 批准号: 6052104
• 负责人: BRUCE M CARLSON
• 金额: $ 31.95万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6052104
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy

This application requests funds to purchase a laser scanning confocal microscope or a group of 17 NIH-funded investigators from several departments at the University of Michigan. The system consists of a Zeiss LSM 510 UV/Visible Confocal Microscope System. The user group is presently sharing its confocal microscopy time on a Bio-Rad MRC-600, with a total of 69 laboratories having used that microscope over the past 12 months. In addition to lack of sufficient access, members of the user group are severely hampered by technical requirements that cannot be met by the existing instrument. Among the needed capabilities are multispectral/brightfield imaging, UV capability, and real-time imaging of living cells. For essentially all of the investigators, 3-D image reconstruction capabilities, a motorized stage and better resolution are important needed features. The Zeiss system was chosen because of its superior UV capabilities and several other technical advantages that are available for little more money than the Bio-Rad MRC 1024, our second choice. Members of the user group have had direct experience with the Zeiss LSM 510 confocal microscope in other institutional settings, and there is no question about its being able to serve our technical needs. In addition, it is a user-friendly instrument with a good maintenance record. The confocal system will be housed within the Cell Biology Laboratories (CBL), a professionally managed University-wide biomedical microscopy core, which is located in the Department of Anatomy and Cell Biology in the Medical School. It is anticipated that members of the user group will be using the new microscope over 90 percent of the time, but provisions have been made for non-group members to have access to the instrument, should free time be available. Policy for the microscope will be determined by an internal advisory committee, and day-by-day maintenance and technical supervision and instruction will be carried out by members of the CBL staff. Ongoing maintenance will be covered through a standard recharge system that is used in the CBL, and long-term continuity is supported by a designated reserve fund for the CBL.

本申请申请资金用于购买激光扫描共聚焦显微镜或由美国国立卫生研究院资助的来自密歇根大学多个部门的17名研究人员。该系统由蔡司LSM 510紫外/可见共聚焦显微镜系统组成。该用户组目前正在分享其在Bio-Rad MRC-600上的共聚焦显微镜时间，在过去的12个月里，共有69个实验室使用了该显微镜。除了缺乏充分的机会外，用户组的成员还受到现有工具无法满足的技术要求的严重阻碍。其中需要的能力是多光谱/明场成像，紫外线能力和活细胞的实时成像。对于所有的研究人员来说，三维图像重建能力、机动化的舞台和更好的分辨率是他们需要的重要特征。选择蔡司系统是因为其优越的紫外线能力和其他几个技术优势，比Bio-Rad MRC 1024多一点钱，我们的第二选择。用户组的成员有直接的经验与蔡司LSM 510共聚焦显微镜在其他机构设置，毫无疑问，它能够满足我们的技术需求。此外，它是一种用户友好的仪器，具有良好的维护记录。共聚焦系统将被安置在细胞生物学实验室（CBL）内，这是一个专业管理的大学范围内的生物医学显微镜核心，位于医学院的解剖和细胞生物学系。预计用户组的成员将在90%以上的时间内使用新显微镜，但如果有空闲时间，非用户组成员也可以使用该仪器。显微镜的政策将由内部咨询委员会决定，日常维护、技术监督和指导将由CBL的工作人员进行。持续的维护将通过CBL中使用的标准补给系统来支付，长期的连续性将由CBL的指定储备基金来支持。