ZEISS LSM 510 CONFOCAL MICROSCOPE SYSTEM

蔡司 LSM 510 共焦显微镜系统

• 批准号: 6052104
• 负责人: BRUCE M CARLSON
• 金额: $ 31.95万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6052104
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy

This application requests funds to purchase a laser scanning confocal microscope or a group of 17 NIH-funded investigators from several departments at the University of Michigan. The system consists of a Zeiss LSM 510 UV/Visible Confocal Microscope System. The user group is presently sharing its confocal microscopy time on a Bio-Rad MRC-600, with a total of 69 laboratories having used that microscope over the past 12 months. In addition to lack of sufficient access, members of the user group are severely hampered by technical requirements that cannot be met by the existing instrument. Among the needed capabilities are multispectral/brightfield imaging, UV capability, and real-time imaging of living cells. For essentially all of the investigators, 3-D image reconstruction capabilities, a motorized stage and better resolution are important needed features. The Zeiss system was chosen because of its superior UV capabilities and several other technical advantages that are available for little more money than the Bio-Rad MRC 1024, our second choice. Members of the user group have had direct experience with the Zeiss LSM 510 confocal microscope in other institutional settings, and there is no question about its being able to serve our technical needs. In addition, it is a user-friendly instrument with a good maintenance record. The confocal system will be housed within the Cell Biology Laboratories (CBL), a professionally managed University-wide biomedical microscopy core, which is located in the Department of Anatomy and Cell Biology in the Medical School. It is anticipated that members of the user group will be using the new microscope over 90 percent of the time, but provisions have been made for non-group members to have access to the instrument, should free time be available. Policy for the microscope will be determined by an internal advisory committee, and day-by-day maintenance and technical supervision and instruction will be carried out by members of the CBL staff. Ongoing maintenance will be covered through a standard recharge system that is used in the CBL, and long-term continuity is supported by a designated reserve fund for the CBL.

该申请要求资金购买激光扫描共焦显微镜或由 17 名来自密歇根大学多个系的 NIH 资助的研究人员组成的团队。 该系统由 Zeiss LSM 510 紫外/可见共焦显微镜系统组成。 用户组目前正在分享其在 Bio-Rad MRC-600 上的共焦显微镜使用时间，过去 12 个月内共有 69 个实验室使用过该显微镜。除了缺乏足够的访问权限之外，用户组成员还受到现有仪器无法满足的技术要求的严重阻碍。 所需的功能包括多光谱/明场成像、紫外线功能和活细胞实时成像。 对于几乎所有研究人员来说，3D 图像重建能力、电动载物台和更好的分辨率是重要的需求特征。 选择蔡司系统是因为其卓越的紫外线功能和其他一些技术优势，而且价格比我们的第二选择 Bio-Rad MRC 1024 贵一点。用户组成员在其他机构环境中直接体验过蔡司 LSM 510 共焦显微镜，毫无疑问它能够满足我们的技术需求。 此外，它还是一款用户友好型仪器，具有良好的维护记录。该共聚焦系统将安置在细胞生物学实验室（CBL）内，这是一个专业管理的全校生物医学显微镜核心，位于医学院解剖学和细胞生物学系。 预计用户组成员将在 90% 以上的时间使用新显微镜，但已规定非组成员在有空闲时间时也可以使用该仪器。显微镜的政策将由内部咨询委员会决定，日常维护以及技术监督和指导将由 CBL 工作人员进行。 持续维护将通过 CBL 使用的标准充值系统进行，并且长期连续性由 CBL 指定的储备基金支持。