MUSCLE REGENERATION AND AGING
肌肉再生和衰老
基本信息
- 批准号:6201004
- 负责人:
- 金额:$ 15.44万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1999
- 资助国家:美国
- 起止时间:1999-08-01 至 2000-06-30
- 项目状态:已结题
- 来源:
- 关键词:Schwann cells age difference aging cell proliferation cell type confocal scanning microscopy denervation electron microscopy electrophysiology histology immunocytochemistry innervation laboratory rat morphology muscle function muscle satellite cell muscle transplantation nervous system regeneration regeneration single cell analysis striated muscles
项目摘要
The overall aims of this Program Project are to test the hypothesis that
denervation changes are responsible for much of the deficit in muscle
mass and function that is seen in old age and to discover enough about
the biology of denervated muscle to be able to devise methods for
successfully preventing or reversing the course of denervation atrophy.
Project 1 will employ principally morphological methods to investigate
both satellite cell biology and to localize in tissue sections important
molecules in muscles at various stages of aging and denervation, as
well as to asses the success of restoration of denervated and/or aging
muscle by grafting or electrical stimulation. In Group Aims 1 and 2,
P-1 will provide quantitative data on the numbers and distribution of
satellite cells on denervated muscle fibers of young and old rats, as
well as localizing by immunocytochemical means molecules of the
myogenic regulatory factor family and the proteins EF-1alpha and S1.
In addition, P-1 will assess morphologically the success of grafts of
denervated and aged muscles into young host rats. In Group Aim 3,
p-1 will use satellite cell analysis and molecular localization techniques
to test the hypothesis that electrical stimulation of denervated muscle
significantly reduces the rate and degree of denervation atrophy. P-1
will also assess the morphology of grafts of stimulated-denervated
muscles in testing the hypothesis that grafts of stimulated-denervated
muscles recover better than grafts of non-stimulated denervated
muscles. For Group aim 4, P-1 will assess the morphology of grafts of
muscles that have been reinnervated with either fast or slow nerves in
young, adult, old and extremely old rats. These studies are expected
to clarify the cellular basis underlying denervation atrophy and the
patterns of recovery seen after different periods of denervation of
muscle. These data will than be used in the analysis of structural and
functional deficits in aging muscle.
本计划项目的总体目标是检验以下假设:
去神经支配的变化是造成肌肉中大部分缺陷的原因。
在老年时期看到的质量和功能,
失神经肌肉的生物学,能够设计出方法,
成功地防止或逆转去神经萎缩的过程。
项目1将主要采用形态学方法来研究
卫星细胞生物学和组织切片定位都很重要
分子在肌肉在不同阶段的老化和去神经,
以及评估失神经和/或老化的恢复的成功性
肌肉移植或电刺激。 在小组目标1和2中,
P-1将提供关于下列人员的人数和分布情况的定量数据:
年轻和老年大鼠失神经肌肉纤维上的卫星细胞,如
以及通过免疫细胞化学方法定位
生肌调节因子家族和蛋白质EF-1 α和S1。
此外,P-1将从形态学上评估移植物的成功率,
去神经和老化的肌肉移植到年轻的宿主大鼠体内。 在目标3组中,
p-1将使用卫星细胞分析和分子定位技术
为了验证电刺激失神经肌肉
显著降低失神经萎缩的速率和程度。 p-1
还将评估移植物的形态刺激去神经
肌肉在测试的假设,刺激去神经移植
肌肉恢复比非刺激失神经移植更好
肌肉. 对于第4组目标,P-1将评估移植物的形态
已经用快神经或慢神经重新支配的肌肉,
年轻、成年、老年和极老年大鼠。 这些研究预计
为了阐明失神经萎缩的细胞基础,
不同时期的失神经支配后的恢复模式,
肌肉. 这些数据将用于分析结构和
老化肌肉的功能缺陷。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
BRUCE M CARLSON其他文献
BRUCE M CARLSON的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('BRUCE M CARLSON', 18)}}的其他基金
SINGLE FIBER ANALYSIS OF DENERVATED AND AGING MUSCLE
去神经支配和老化肌肉的单纤维分析
- 批准号:
2863149 - 财政年份:2000
- 资助金额:
$ 15.44万 - 项目类别:
相似海外基金
Sex and age difference in the immune response to viral myocarditis
病毒性心肌炎免疫反应的性别和年龄差异
- 批准号:
440151 - 财政年份:2020
- 资助金额:
$ 15.44万 - 项目类别:
Studentship Programs
An fMRI study of the effect of age difference on mind attribution
年龄差异对心理归因影响的功能磁共振成像研究
- 批准号:
19J12925 - 财政年份:2019
- 资助金额:
$ 15.44万 - 项目类别:
Grant-in-Aid for JSPS Fellows
Effects of traumatic brain injury on hippocampal network activity: age difference
创伤性脑损伤对海马网络活动的影响:年龄差异
- 批准号:
8443632 - 财政年份:2013
- 资助金额:
$ 15.44万 - 项目类别:
Effects of traumatic brain injury on hippocampal network activity: age difference
创伤性脑损伤对海马网络活动的影响:年龄差异
- 批准号:
8669899 - 财政年份:2013
- 资助金额:
$ 15.44万 - 项目类别:
Subsurface water mass variations in the Kuroshio region inferred from 14C age difference of planktic foraminifers with different depth habitat
不同深度栖息地浮游有孔虫14C年龄差异推断黑潮地区地下水质量变化
- 批准号:
22654061 - 财政年份:2010
- 资助金额:
$ 15.44万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
AGE DIFFERENCE IN ATTENTION--CONSEQUENCES FOR MEMORY
注意力的年龄差异——对记忆力的影响
- 批准号:
3453621 - 财政年份:1992
- 资助金额:
$ 15.44万 - 项目类别:
AGE DIFFERENCE IN ATTENTION--CONSEQUENCES FOR MEMORY
注意力的年龄差异——对记忆力的影响
- 批准号:
2051816 - 财政年份:1992
- 资助金额:
$ 15.44万 - 项目类别:
AGE DIFFERENCE IN ATTENTION--CONSEQUENCES FOR MEMORY
注意力的年龄差异——对记忆力的影响
- 批准号:
2051814 - 财政年份:1992
- 资助金额:
$ 15.44万 - 项目类别:
AGE DIFFERENCE IN ATTENTION--CONSEQUENCES FOR MEMORY
注意力的年龄差异——对记忆力的影响
- 批准号:
3453620 - 财政年份:1992
- 资助金额:
$ 15.44万 - 项目类别: