EPICS XL-MCL FLOW CYTOMETRY SYSTEM

EPICS XL-MCL 流式细胞术系统

• 批准号: 6052297
• 负责人: FRED S. ROSEN
• 金额: $ 11.1万
• 依托单位: IMMUNE DISEASE INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6052297
• 关键词: biomedical equipment purchase; flow cytometry

The objective of this proposal is to acquire an analytical flow cytometry which will be shared by investigators at the Center for Blood Research (CBR) to conduct established and new research projects focused on various aspects of blood research. The current projects that will specifically utilize this instrument are: 1) a three-component program project focused on blood cell signaling pathways and cytoskeletal organization (Dr. Fred S. Rosen), (2) studies of the genetic and cellular mechanisms of immunity and host defense (Dr. Chester A. Alper), (3) studies of T lymphocyte function in HIV infection disease, the Wiskott- Aldrich syndrome (Dr. Eileen Remold-O-Donnell). Analytical flow cytometry us a central technical approach for all of this projects, which are funded by NIH-peer received grants, and will be used in a variety of cell and molecular biological model systems to investigate the function of blood cell surface and intracellular proteins. The results derived from these ongoing investigations will lead to a better understanding of the molecular mechanisms involved in immunity, host defense and cell differentiation and will provide fundamental information on the mechanisms that control key immunological and hematological functions. The requested Coulter Model XL flow cytometry analyzer system will enhance the productivity of these established and expanding research projects. This instrument is state-of-the-art and has features that meet the growing applications by blood research scientists to studies of the structure and function of blood cells by versatile and refined flow cytometry techniques. This instrument is capable of analyzing up to four differently labeled fluorescent probes in a single sample which increases the accuracy and decreases the cost, time and sample size required for analysis. The IBM compatible acquisition and analysis computer programs will allow both automated, high throughput analyses and specialized, refined advanced applications such as are required for kinetic measurements and quantitations of intracellular antigens.

该提案的目的是获得一种分析流式细胞仪，该分析流式细胞仪将由血液研究中心（CBR）的研究人员共享，以开展专注于血液研究各个方面的既定和新的研究项目。目前的项目，将专门利用这一工具是：1）一个三部分的计划项目，重点是血细胞信号通路和细胞骨架组织（博士弗雷德S。罗森），（2）免疫和宿主防御的遗传和细胞机制的研究（Chester A. Alper），（3）HIV感染疾病中T淋巴细胞功能的研究，Wiskott-Aldrich综合征（Eileen Remold-O-Donnell博士）。分析流式细胞术是所有这些项目的核心技术方法，这些项目由NIH同行赠款，并将用于各种细胞和分子生物学模型系统，以研究血细胞表面和细胞内蛋白质的功能。从这些正在进行的研究中得出的结果将导致更好地了解参与免疫，宿主防御和细胞分化的分子机制，并将提供控制关键免疫和血液功能的机制的基本信息。所要求的库尔特XL型流式细胞仪系统将提高这些已建立和正在扩大的研究项目的生产力。该仪器是最先进的，具有满足血液研究科学家通过多功能和精细的流式细胞术技术研究血细胞结构和功能的日益增长的应用的功能。该仪器能够在单个样品中分析多达四种不同标记的荧光探针，这提高了准确性并降低了分析所需的成本、时间和样品大小。IBM兼容的采集和分析计算机程序将允许自动化、高通量分析和专门化、精细化的高级应用，例如细胞内抗原的动力学测量和定量所需的应用。