PURCHASE OF ELECTRON PARAMAGNETIC RESONANCE EQUIPMENT

电子顺磁共振设备采购

• 批准号: 6053120
• 负责人: WAYNE L HUBBELL
• 金额: $ 37.24万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6053120
• 关键词: biomedical equipment purchase; electron spin resonance spectroscopy

Electron paramagnetic resonance (EPR) spectroscopy is a powerful tool for the characterization of proteins both by introduction of a spin label probe and by characterization of metal binding sites. Although the NIH supported researchers at UCLA who utilize this technique are spread out in several different departments, there is a long history of sharing of the available equipment as evidenced by many joint publications resulting from these fruitful collaborations. A Center for Biological EPR has been recently established to improve the visibility of the available EPR resources for the benefit of other potential users. At this time, there is a critical need to upgrade much of the very old EPR instrumentation that has supported research in this area for many years. The research projects of three NIH supported major users are described with the following titles: l) Structure and Dynamics of Proteins Using Site-Directed Spin Labeling; 2) Structure-Function Studies on the Lactose Permease of Escherichia coli using Electron Paramagnetic resonance; and 3) Electron Paramagnetic Resonance (EPR) Studies on the Structure and Function of FALS-Mutant Proteins of Copper-Zinc Superoxide Dismutase. Five additional NIH supported researchers are potential users of the requested instrumentation.

电子顺磁共振（EPR）光谱是一个强大的工具，用于表征蛋白质的引入自旋标记探针和金属结合位点的表征。虽然美国国立卫生研究院支持的研究人员在加州大学洛杉矶分校谁利用这种技术分布在几个不同的部门，有一个共享的可用设备的悠久历史，从这些富有成效的合作产生的许多联合出版物证明。最近成立了一个生物EPR中心，以提高现有EPR资源的可见度，使其他潜在用户受益。在这个时候，有一个关键的需要，以升级许多非常旧的EPR仪器，支持在这一领域的研究多年。美国国立卫生研究院支持的三个主要用户的研究项目描述如下：1）使用定点自旋标记的蛋白质结构和动力学; 2）使用电子顺磁共振对大肠杆菌乳糖渗透酶的结构-功能研究; 3）铜锌超氧化物歧化酶的突变蛋白的结构和功能的电子顺磁共振（EPR）研究。另外五名NIH支持的研究人员是所要求仪器的潜在用户。