PURCHASE OF ELECTRON PARAMAGNETIC RESONANCE EQUIPMENT

电子顺磁共振（EPR）光谱是一种强大的工具，可以通过引入自旋标记探针和表征金属结合位点来表征蛋白质。尽管美国国立卫生研究院支持的加州大学洛杉矶分校的研究人员利用这种技术分散在几个不同的部门，但从这些富有成效的合作产生的许多联合出版物来看，共享可用设备的历史很长。最近建立了一个生物EPR中心，以提高现有EPR资源的可见性，从而使其他潜在用户受益。目前，迫切需要升级许多非常旧的EPR仪器，这些仪器多年来一直支持该领域的研究。三个NIH支持的主要用户的研究项目描述如下：1)使用位点定向自旋标记的蛋白质结构和动力学；2)利用电子顺磁共振技术研究大肠杆菌乳糖渗透酶的结构-功能；3)铜锌超氧化物歧化酶fals突变蛋白结构和功能的电子顺磁共振（EPR）研究。另外五名NIH支持的研究人员是所要求仪器的潜在用户。

Electron paramagnetic resonance (EPR) spectroscopy is a powerful tool for the characterization of proteins both by introduction of a spin label probe and by characterization of metal binding sites. Although the NIH supported researchers at UCLA who utilize this technique are spread out in several different departments, there is a long history of sharing of the available equipment as evidenced by many joint publications resulting from these fruitful collaborations. A Center for Biological EPR has been recently established to improve the visibility of the available EPR resources for the benefit of other potential users. At this time, there is a critical need to upgrade much of the very old EPR instrumentation that has supported research in this area for many years. The research projects of three NIH supported major users are described with the following titles: l) Structure and Dynamics of Proteins Using Site-Directed Spin Labeling; 2) Structure-Function Studies on the Lactose Permease of Escherichia coli using Electron Paramagnetic resonance; and 3) Electron Paramagnetic Resonance (EPR) Studies on the Structure and Function of FALS-Mutant Proteins of Copper-Zinc Superoxide Dismutase. Five additional NIH supported researchers are potential users of the requested instrumentation.