FACSCALIBUR FLOW CYTOMETER SYSTEM

分子医学和免疫学中心(CMMI)的主要工作重点是癌症、患者和动物模型的免疫诊断和免疫治疗。在该中心进行的单个项目都需要流式细胞仪的支持。该中心有一台Becton Dickinson FACScan，它是1989年购买的。在过去的十年里，该中心的所有调查人员一直在大量使用该仪器。在购买FACScan以来的十年里，流式细胞仪技术有了很大的进步，使今天可用的仪器在生产率和性能方面都要优越得多。此外，在这段时间里，我们对流式细胞仪的需求也在增长。因此，我们提交了这份申请，要求收购FACSCaliber Flow Cytometer系统和FACSLoader，这是一种更新的流式细胞仪系统，将具有更高的可靠性、效率和更广泛的科学应用。该中心的流式细胞术的具体应用包括：淋巴细胞表型，筛选细胞系与单抗的反应性，根据与肿瘤细胞系和/或正常细胞的反应性或无反应性来选择单抗，DNA细胞周期分析，细胞凋亡研究，干细胞制剂中肿瘤细胞的检测，树突状细胞的分析，以及用于治疗研究中毒性评估的绝对细胞计数。对仪器的行政/科学监督，包括对内部工作人员的日常管理和技术培训，将由斯坦博士负责，并在该中心基础研究委员会的建议下进行。我们估计，主要用户将需要仪器总使用量的大约75%-80%。此外，该仪器将提供给CMMI内部不太频繁的用户以及CMMI之外的用户，包括距离CMMI很近的罗格斯大学纽瓦克生物科学系的成员。

A major focus of the work at the Center for Molecular Medicine and Immunology (CMMI) is immunodiagnosis and immunotherapy of cancer, in patients and in animal models. The individual projects performed at the Center all require flow cytometry support. The Center has a Becton Dickinson FACScan, which was purchased in 1989. The instrument has received continuous heavy usage over the past decade by all investigators at the Center. During the ten years since the FACScan was purchased flow cytometry technology has advanced considerably, making the instrument available today much superior in terms of productivity and performance. In addition, our needs for flow cytometry have grown during this time period. Thus, we are submitting this application for the acquisition of the FACSCaliber Flow Cytometer System with the FACSLoader, an updated flow cytometry system, which will have greater reliability, efficiency, and broader scientific applications. Specific uses of flow cytometry at the Center include: lymphocyte phenotyping, screening cell lines for reactivity with MAbs, selection of MAbs based on reactivity or lack of reactivity with tumor cell lines and/or normal cells, DNA cell cycle analysis, apoptosis studies, detection of tumor cells in stem cell preparations, analyses of dendritic cells, and absolute cell counting for toxicity evaluations in therapy studies. Administrative/scientific oversight for the instrument, including day-to- day management and technical training for in-house staff will be the responsibility of Dr. Stein, under advisement of the Center's Basic Research Committee. We estimate that the major users will require approximately 75-80% of the total usage of the instrument. In addition, the instrument will be available to less frequent users within CMMI as well as users outside of CMMI, including members of the Biological Science Department at Rutgers Newark which is located a short distance from CMMI.