MOFLO TM MULTI-LASER FLOW CYTOMETER

该申请是为了获得资金，用于在康涅狄格大学健康中心（UCHC）购买新的荧光激活细胞分选仪。该仪器，MoFlo by Cytomation，将成为已经高度活跃的核心流式细胞术设施的一部分。我们目前的文书已经过时，也无法跟上需求。这在一定程度上是由于最近和正在进行的招聘NIH资助的教师。在过去的两年里，UCHC创建了三个新的部门/中心：免疫治疗中心，微生物发病中心以及遗传学和发育生物学系。所有三位中心主任都积极参与流式细胞术，是这项提案的一部分：遗传学新主席Marc Lalande博士在流式细胞术（例如染色体分选）方面有丰富的经验，是该设施的宝贵资产。此外，每个中心将招募几个新的教师，其中许多将需要流式细胞术在他们的研究。我们最近还招募了一名干细胞免疫学家（H. L。与我一起训练的阿吉拉。Weissman，斯坦福大学），该研究正在等待NIH的资助，并且需要大量使用MoFlo。此外，除了新的实验室，其他几个NIH资助的团体也有新的流式细胞术技术的基本需求。如申请中所述，MoFlo将允许比UCHC目前可用的更快速和有效的分选以及更复杂的分析能力。这反过来将增加我们正在进行的和未来的NIH资助项目的成功机会。

This application is to obtain funding for the purchase of a new fluorescence activated cell sorter at the University Of Connecticut Health Center (UCHC). This instrument, the MoFlo by Cytomation, would become part of an already highly active core flow cytometry facility. Our present instrument is outdated and also unable to keep up with demand. This is due in part to the recent and ongoing recruitment of NIH-funded faculty. Three new Departments/Centers have been created at UCHC in the last two years: the Center for Immunotherapy, the Center for Microbial Pathogenesis, and the Department Of Genetics and Developmental Biology. All three of the center directors are heavily involved in flow cytometry and are part of this proposal: Dr. Marc Lalande, new Chair of Genetics, has much experience in flow cytometry (e.g. sorting of chromosomes) and is a valuable asset to the facility. In addition, each center will be recruiting several new faculty, and many of these will require flow cytometry in their research. We have also recently recruited a stem cell immunologist (H.L. Aguila who trained with I. Weissman, Stanford) who has pending NIH funding and who will require significant use of the MoFlo. Furthermore, besides the new laboratories, several other NIH funded groups also have essential needs for new flow cytometry technology. As described in the application, the MoFlo will allow much more rapid and efficient sorting and more complex analytical power than is presently available at UCHC. This in turn will increase the chances of success for our ongoing and future NIH funded programs.