ENERGY TRANSFER IN BLUE LIGHT PHOTORECEPTOR

我们最近分离出了一种蓝光感光蛋白。 拟南芥(植物)；这是第一个这样的光感受器 在任何生物体中都能被识别。光感受器在结构上看起来 类似于微生物光解酶(DNA修复酶)。光解酶 结合两个发色团；其中一个(亚甲基四氢叶酸)起作用 作为另一种(催化)黄油的捕光天线 生色团。最近已经确定，蓝光 光感受器类似地结合两个发色团。因此，它是 我们研究的目标是利用RLBL的设施来确定 在光感受器中观察到的类型的能量转移是否 学习。一旦在中建立了能量转移的参数 野生型感光蛋白，我们将提供突变体 光感受器蛋白已被生物学确定为 处于非活动状态。关于野生型和突变型蛋白的数据应该提供 对我们小说中动作手法的宝贵洞察 光感受器，并可能阐明生色团间能量的特征 将具有普遍关联性的转让。我们目前正在研究 这些系统的光稳定性。

We have recently isolated a blue light photoreceptor protein from Arabidopsis thaliana (plants); this is the first such photoreceptor to be identified in any organism. The photoreceptor appears structurally similar to microbial photolyases (DNA repair enzymes). Photolyases bind two chromophores; one of which (methenyl tetrahydrofolate) serves as a light-harvesting antenna for the other (catalytic) flavin chromophore. It has recently been determined that the blue light photoreceptor similarly binds two chromophores. It is accordingly the goal of our study to utilize the facilities of the RLBL to determine whether energy transfer of the type observed in photoreceptor for such studies. Once the parameters for energy transfer are established in the wild-type photoreceptor protein, we will supply mutant photoreceptor proteins which have been determined to be biologically inactive. Data on both wild-type and mutant proteins should supply valuable insight into the mec hanism of action of our novel photoreceptor and may illuminate features of inter-chromophore energy transfer that will have general relevance. We are presently studying the photostability of these systems.