ENDONUCLEASE PROTEIN FEN 1 STUDIES

小角x射线散射研究将在一个 核酸内切酶蛋白(FEN-1)。这种蛋白质在DNA中很重要 复制和损坏修复。它识别特定类型的 DNA中的分支缺陷，它专门修复。DNA结合 修复和修复似乎是这种蛋白质的独立属性。我们也 有仍能结合DNA但已失去修复能力的突变体 才能。此外，修复需要镁的存在。我们打算 检测4个样本，野生型(/-mg)和修复失活 突变(/-mg)。高通量同步加速器束和足够的 低背景SAXS设施是获取足够数据所必需的 这些稀溶液样本的统计数据(约1.5 mg/ml)。

Small angle x-ray scattering studies will be performed on an endonuclease protein (FEN-1). This protein is important in DNA replication and damage repair. It recognizes a specific type of branch defect in the DNA which it specifically repairs. DNA binding and repair seem to be separate properties of this protein. We also have mutants which can still bind DNA but have lost their repair ability. Also, repair requires magnesium to be present. We intend to measure 4 samples, the wild type (+/- Mg) and the repair inactivated mutant (+/- Mg). A high-flux synchrotron beam and an adequate low-background SAXS facility are necessary to get enough data statistics from these dilute solution samples (approx. 1.5 mg/ml).