Neurokinetics Multiaxes Vestibular Test System

神经动力学多轴前庭测试系统

• 批准号: 6441106
• 负责人: Stephen Morris Highstein
• 金额: $ 29.59万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6441106
• 关键词: assistive device /technology; behavior test; biomedical equipment purchase; diagnosis design /evaluation; eye movements; genetically modified animals; laboratory mouse; mutant; nervous system disorder diagnosis; neurofilament; vestibular apparatus; vestibular nerve; vestibuloocular reflex

A complete vestibular evaluate system including a multi axis vestibular motion system, an eye movement measurement system, an animal stabilization device, a single unit amplifier, and a data acquisition system is requested. The instrument will be utilized by each of the Principle investigators to evaluate vestibular function. The instrument is capable of performing behavioral tests including linear and angular acceleration and off vertical rotary testing. Some investigator and the transgenic core will examine vestibular-evoked eye movements and others will determine the response dynamics of single vestibular afferent nerve fibers. The unit will be housed within the Old Shriner's Hospital at Washington University one floor above the transgenic mouse core and will also serve as a screening device for vestibular deficits in mutant mice. The humans who complain of subjective vertigo or balance difficulties objective vestibular deficits are often subtle and can only be revealed by behavioral tests in a clinical laboratory setting. Eye movements have been extremely useful in this regard. For example, nystagmus produced during head positioning tests is diagnostic for benign positional paroxysmal vertigo and head-thrusting tests can reveal subtle specific semicircular canal dysfunction. Mice lack the capability of speech; therefore if we hope to provide mouse models for specific vestibular defects it is necessary to implement sophisticated tests to document these defects. We desire to standardized the vestibulo-ocular responses to angular and linear acceleration in the mouse. To that end we need a device that can provide specified angular and linear acceleration as well as off vertical axis rotation. Such a platform will enable the evaluation of vestibulo-ocular reflexes over the entire relevant range of frequencies and amplitudes. Further, in the likely scenario of the necessity to test specific therapeutic agents a normative database which to evaluate the action of pharmacological agents will be invaluable. Existing tests such as the vestibular evoked response are not nearly as sensitive as neural recording.

需要一个完整的前庭评估系统，包括多轴前庭运动系统、眼动测量系统、动物稳定装置、单单元放大器和数据采集系统。该仪器将被每个主要调查员用来评估前庭功能。该仪器能够进行包括线性和角加速度以及非垂直旋转测试在内的行为测试。一些研究者和转基因核心将检查前庭诱发的眼球运动，其他人将确定单个前庭传入神经纤维的反应动力学。该装置将被安置在华盛顿大学的老施莱纳医院，在转基因小鼠核心的一层楼上，也将作为筛查突变小鼠前庭功能缺陷的设备。主观性眩晕或平衡困难的人，客观前庭功能缺陷往往是微妙的，只能通过临床实验室设置的行为测试来揭示。在这方面，眼球运动非常有用。例如，头部定位试验时产生的眼球震颤可诊断良性阵发性眩晕，头部推力试验可显示细微的特定半规管功能障碍。老鼠缺乏语言能力；因此，如果我们希望为特定的前庭缺陷提供小鼠模型，就有必要实施复杂的测试来记录这些缺陷。我们希望标准化前庭眼对角加速度和线加速度的反应。为此，我们需要一种能够提供特定角加速度和线加速度以及离垂直轴旋转的装置。这样一个平台将能够评估整个相关频率和幅度范围内的前庭-眼反射。此外，在可能需要测试特定治疗药物的情况下，评估药理学药物作用的规范数据库将是非常宝贵的。现有的测试，如前庭诱发反应，远不如神经记录灵敏。