NEURAL MECHANISMS OF VESTIBULAR FUNCTION IN FISH

该提案的总体目标是确定决定因素的特征 水平半规管的反应动力学 前庭传出系统的机制及后遗症研究 操纵这些决定因素。的响应动力学。 水平半规管神经已在该物种中被记录。 根据他们的反应，有三大类传入 研究了整个频率和幅度频谱的动力学。确实有 中表示的依赖于频率和幅度的非线性 某些类别的传入反应。这些贡献和 假设的中心和中心的微分运动的意义 毛细胞感受器电流频率和毛细胞的周围 初级传入神经元的幅值相关非线性 将会被评估。全细胞膜片钳技术将应用于 从确定的眉骨功能区分离的毛细胞到 研究激活、失活和 钾电流失活。电流钳位响应 这些电池的扰动电流将被确定。差动 不同类别的初级传入的传出动作也 已被记录在案。这种差异的含义和机制 将用形态和生理技术来研究它们的作用。 从初级记录的微型(M)EPSP的幅度和形状 在刺激传出神经之前和期间将测量传入神经。 评价EPSP传出诱发改变的意义 特性到系统的输出。的激活后遗症 游离传出前庭系统对水平神经管传入的影响 将利用多通道电极研究游动的鱼，在 水声遥测和水下视频。因此，我们希望到达 在这个物种中传出行为的一般理论。对中国传统文化的研究 前庭系统及其相关的细胞和系统科学方面 传出控制添加有关功能的信息，并可能与 未来可能的运动控制疗法和机制 生病了。

The overall goals of the proposal are to characterize the determinants of the response dynamics of the horizontal semicircular canal and to study the mechanisms and sequels of the efferent vestibular systems manipulation of these determinants. The response dynamics of the horizontal semicircular canal nerve have been documented in this species. There are three broad classes of afferent based upon their response dynamics across the frequency and amplitude spectrum studied. There are frequency and amplitude dependent non-linearities represented within the responses of certain classes of afferent. The contributions and significance of the putative differential motion of the center and periphery of the cupula and of hair cell receptor current frequency and amplitude dependent non-linearities to those in the primary afferents will be evaluated. Whole cell patch clamp techniques will be applied to hair cells isolated from defined functional regions of the crista to study the magnitude and kinetics of activation, inactivation, and deactivation of potassium currents. The current clamp responses to perturbing currents of these cells will be determined. Differential efferent action upon the various classes of primary afferent has also been documented. The implications and mechanisms of this differential action will be studied with morphological and physiological techniques. The amplitudes and shapes of miniature (m) EPSPs recorded from primary afferents will be measured before and during stimulation of efferents to assess the significance of the efferent evoked changes in EPSP characteristics to the output of the system. The activation sequelae of the efferent vestibular system upon horizontal canal afferents in free swimming fish will be studied utilizing multi-channel electrodes, under water acoustic telemetry and under water video. Thus, we hope to arrive at a general theory of efferent action in this species. Studies of the cellular and systems science aspects of the vestibular system and its efferent control add information about function and may bear upon possible future therapies and mechanisms for the control of motion sickness.