CORE--COMPUTER AND PERIPHERALS

核心——计算机及外设

• 批准号: 6448949
• 负责人: Stephen Morris Highstein
• 金额: $ 16.33万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6448949
• 关键词: biomedical facility; computer network; computers

In this project we will seek to resolve the origin of differences in tonic and phasic response output from the hair cell and cupula of the vestibular system. Specifically, we will determine if differences in encoded vestibular output is attributable to: (1) cupular motion and regional variation in hair cell stereociliary deflection; (2) afferents that are differentially (numerically and/or regionally) innervated by hair cells; and/or (3) that afferents may respond differentially to a transmitter quantum. This will be accomplished through a coordinated interdisciplinary effort encompassing digital video imaging image analysis, detailed electrophysiological recording and kinetic simulation studies described throughout this program project application. Silver's laboratory will focus upon the acquisition and analysis of images of stereociliary and cupular movements during head displacement, by developing a digital video light microscopic method for directly observation, analysis and relating movements of sub-cellular, cellular and supra-cellular structures in the vestibular end organ (VEO) related with vestibular function, using the toadfish (Opsanus tau) labyrinth as the model system, Reeves' group will focus upon advanced analytical imaging methods. High quality imagery of the performance of VEO components is needed to establish the structure- function relationships of these essential mechano-electrical transducers, especially in light of the advances made by the collaborating laboratories in hair cell electrophysiology and computational displacements of these transducers obtained on-line during an actual experiment is lacking. Recently, Silver, in collaboration with Highstein, accomplished direct video light microscopic imaging microscope capable of video observation and recording of stereociliary and cupular movements in situ in concert with concurrent electrophysiological recordings; (2) describe the sub-micrometer displacements of these structures during VEO movements via computational representations of image data (i.e., processed imagery, isosurfaces and isovolume) to aid in testing for the parity correspondence among actual VEO movements (this work), electrophysiological performance (Highstein and Boyle) and kinetic simulations (Rabbit, Miller). Integration of the information developed will facilitate assessment of normal and dysfunctional VEO performance in normal, and microgravity environments.

在这个项目中，我们将寻求解决分歧的根源 从毛细胞和丘脑输出的强直和时相反应 前庭系统。具体地说，我们将确定在 编码的前庭输出可归因于：(1)杯状运动和 毛细胞立体纤毛偏转的区域变异；(2)传入 以不同方式(在数字上和/或区域上)由 毛细胞；和/或(3)传入细胞可能对 发射机量子。这将通过协调一致的 包括数字视频成像图像在内的跨学科工作 分析、详细的电生理记录和动力学模拟 研究描述贯穿整个计划项目的应用。西尔弗的 实验室将专注于获取和分析 头部移位期间的立体纤毛和杯状运动，由 一种数字视频光镜直接成像方法的研制 观察、分析和关联亚细胞、细胞运动 与前庭末梢器官(VEO)相关的超细胞结构 具有前庭功能，使用蟾鱼迷路作为 模型系统，里夫斯的团队将专注于高级分析 成像方法。VEO性能的高质量图像 建立结构-功能关系需要组件 这些基本的机电换能器，特别是在光线下 毛细胞方面的合作实验室取得的进展 这些换能器的电生理学和计算位移 缺乏在实际实验中在线获取的数据。最近， Silver与Highstein合作，实现了直射视频光线 可视频观察和记录的显微成像显微镜 立体纤毛和杯状运动的原位同步 电生理记录；(2)描述亚微米 这些结构在VEO运动过程中的位移通过计算 图像数据的表示(即，处理后的图像、等值面和 等体积)来帮助测试实际数据之间的奇偶对应 Veo运动(本作品)、电生理性能(Highstein 和动力学模拟(Rabbit，Miller)。整合了 开发的信息将有助于评估正常和 在正常和微重力环境下VEO表现异常。