ASSEMBLY AND STRUCTURE OF BACTERIOPHAGE LAMBDA

噬菌体 Lambda 的组装和结构

• 批准号: 6386335
• 负责人: ROGER W HENDRIX
• 金额: $ 46.75万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-12-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6386335
• 关键词: Escherichia coli; X ray crystallography; bacteriophage lambda; cryoelectron microscopy; gene mutation; protein structure; protein structure function; site directed mutagenesis; virus assembly; virus genetics; virus protein; virus replication

The dsDNA bacteriophages provide an unparalleled resources for investigating the principles of biological assembly at the molecular level. This is a proposal to continue the study of these problems using the E. coli bacteriophages lambda and HK97. Specific interests include assembly of the heads of HK97, with an emphasis on the extensive conformational rearrangement in the capsid protein that happens during DNA packaging assembly of the phage lambda tail, with an emphasis on a newly discovered chaperone-mediated step in assembly of the tail length template, and assembly and structure of the lambda long tail fibers. Information gained in this study will be generalizable beyond bacteriophages and will therefore affect our understanding of, among others, assembly processes in animal and plant viruses, which are possible points for anti-viral intervention.

双链 DNA 噬菌体为研究分子水平上的生物组装原理提供了无与伦比的资源。这是使用大肠杆菌噬菌体 lambda 和 HK97 继续研究这些问题的建议。具体兴趣包括 HK97 头部的组装，重点是噬菌体 lambda 尾的 DNA 包装组装过程中衣壳蛋白中发生的广泛构象重排，重点是尾长模板组装中新发现的分子伴侣介导的步骤，以及 lambda 长尾纤维的组装和结构。这项研究中获得的信息将适用于噬菌体之外的领域，因此将影响我们对动植物病毒组装过程等的理解，而这可能是抗病毒干预的要点。