ASSEMBLY AND STRUCTURE OF BACTERIOPHAGE LAMBDA

dsDNA噬菌体为在分子水平上研究生物组装的原理提供了无与伦比的资源。这是一个建议，继续研究这些问题，使用E。大肠杆菌噬菌体λ和HK97。具体的利益包括组装的头部HK97，强调广泛的构象重排的衣壳蛋白，发生在DNA包装组装的噬菌体λ尾，强调一个新发现的分子伴侣介导的步骤，在组装的尾长模板，组装和结构的λ长尾纤维。在这项研究中获得的信息将是一般化的超越噬菌体，因此将影响我们的理解，除其他外，在动物和植物病毒，这是抗病毒干预的可能点组装过程。

The dsDNA bacteriophages provide an unparalleled resources for investigating the principles of biological assembly at the molecular level. This is a proposal to continue the study of these problems using the E. coli bacteriophages lambda and HK97. Specific interests include assembly of the heads of HK97, with an emphasis on the extensive conformational rearrangement in the capsid protein that happens during DNA packaging assembly of the phage lambda tail, with an emphasis on a newly discovered chaperone-mediated step in assembly of the tail length template, and assembly and structure of the lambda long tail fibers. Information gained in this study will be generalizable beyond bacteriophages and will therefore affect our understanding of, among others, assembly processes in animal and plant viruses, which are possible points for anti-viral intervention.