CORE--DNA ANALYSIS

核心——DNA分析

• 批准号: 6436432
• 负责人: JANICE A NICKLAS
• 金额: $ 7.89万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-08-01 至 2005-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6436432
• 关键词: biomedical facility; nucleic acid sequence; polymerase chain reaction; single strand conformation polymorphism

Description: (Applicant's Description) The VCC DNA Analysis Core Facility provides fast, inexpensive, user-friendly DNA analysis services to the members of the Vermont Cancer Center. Primary services offered include DNA sequencing, DNA fragment analysis and real-time quantitative PCR. The Facility contains an ABI 373XL automated sequencer with stretch plate capability, 48 lane upgrade and a recent (September, 1999) filter wheel upgrade to enable state-of-the-art chemistry, enhanced run length and optimal signal to noise ratio for sequence runs. The facility also contains an ABI 310 Prism Genetic Analyzer. Wet laboratory dedicated to the facility is approximately 300 s.f. within VCC laboratories in the Medical Alumni Facility. In addition to the labs, an adjacent office is used for sample intake and high-performance user workstations enabling clients to conduct data review and analyses on-site with special software and with direct access to facility staff (software including Genotyper, Sequence Navigator, AutoAssembler, DNASTAR, Primer Express and Sequencher). A Luminex 100 instrument was acquired in April 1999 enabling high-throughput DNA hybridizations (or antigen-antibody or ligand-receptor reactions) on styrofoam beads. In October 1999 the Facility acquired an ABI 7700 (TaqMan) instrument to allow real-time quantitative PCR experiments to be performed by VCC members. The Facility performs over 10,000 lanes of sequence per year and over 8,000 cycle sequencing reactions. Over 80 percent of the cycle sequencing reactions and over 65 percent of the lanes of sequence performed are for VCC members in support of qualifying peer-reviewed studies. The Facility performs other important functions in support of VCC research including: sponsoring seminars and Open House events, tutorials, obtaining bulk prices on reagents, and providing troubleshooting or assistance designing experiments utilizing new technologies. The Facility is user-friendly and responsive to VCC member needs and requests, including new techniques and technologies; a User Group meets quarterly with the facility Director, Manager and VCC Administrator to provide feedback on services, costs, availability, and need for new technologies or instrumentation. Mr. Hunter, the Facility Manager, chairs the VCC Shared Resource Committee and is a member of the VCC Executive Committee.

描述：（申请人的描述）VCC DNA分析核心设施 为会员提供快速、廉价、用户友好的DNA分析服务 佛蒙特州癌症中心提供的主要服务包括DNA 测序、DNA片段分析和实时定量PCR。的 设施包含ABI 373 XL自动测序仪（带拉伸板） 容量，48通道升级和最近（1999年9月）的滤光轮 升级以实现最先进的化学工艺、更长的运行时间和最佳的 序列运行的信噪比。该设施还包含ABI 310棱镜基因分析仪。湿实验室专用于该设施是 在医学校友设施的VCC实验室内约300平方英尺。 除了实验室外，相邻的办公室用于样本采集， 高性能用户工作站，使客户能够进行数据审查， 使用特殊软件和直接访问设施进行现场分析 工作人员（软件包括Genotyper，Sequence Navigator，AutoAssembler， DNASTAR，Primer Express和Sequencher）。使用Luminex 100仪器， 1999年4月获得，能够进行高通量DNA杂交（或 抗原-抗体或配体-受体反应）。十月 1999年，该设施获得了ABI 7700（TaqMan）仪器， 由VCC成员进行定量PCR实验。该设施 每年执行超过10，000个序列通道和超过8，000个周期 测序反应超过80%的循环测序反应， 超过65%的序列通道是为VCC成员执行的， 支持合格的同行评审研究。 该基金还履行其他重要职能，支持风险资本研究 包括：赞助研讨会和开放日活动，教程，获得 批量价格的试剂，并提供故障排除或协助设计 利用新技术的实验。该设施方便用户使用， 响应VCC成员的需求和要求，包括新技术和 技术;用户组每季度与设施主任、经理 和VCC管理员提供有关服务、成本、可用性的反馈， 以及对新技术或仪器的需求。亨特先生， 经理，担任VCC共享资源委员会主席，是VCC成员 执行委员会。