Projections from the amygdala to the cortex: an EM study

从杏仁核到皮质的投射：一项电磁研究

• 批准号: 6340360
• 负责人: JENNIFER L FREESE
• 金额: $ 2.3万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6340360
• 关键词: Macaca mulatta; amygdala; brain electrical activity; brain imaging /visualization /scanning; calcium binding protein; electron microscopy; interneurons; neocortex; neural information processing; neuroanatomy

The objective of this project is to describe, at the electron microscopic level ,the connection between the amygdala and the visual neocortex of non-human primates. The amygdala is essential for emotional response, and it may be involved in a variety of illnesses, including epilepsy and autism. While it known that the amygdala and the cortex are heavily interconnected, little is known concerning the synaptic organization of these connections. The anterograde tracer, Phaseolus vulgarie- leucoagglutinin (PHA-L), will be stereotaxically injected into the amygdala of adult male rhesus monkeys. The transported tracer will under the electron microscope to characterize the projections from the amygdala to area TE in the inferior temporal gyrus and VI in the occipital cortex. The former cortical areas is involved in facial processing and is known to receive strong projections from the amygdala. In second phase of the study, tissue will be double-labeled with an antibody to parva

该项目的目标是在电子显微镜水平上描述非人类灵长类动物的杏仁核和视觉新皮质之间的联系。杏仁核对情绪反应至关重要，它可能与多种疾病有关，包括癫痫和自闭症。虽然人们知道杏仁核和大脑皮层紧密相连，但对这些联系的突触组织却知之甚少。顺行示踪剂，菜豆亮氨酸凝集素(PHA-L)，将被立体定向注射到成年雄性恒河猴的杏仁核中。被运送的示踪剂将在电子显微镜下表征从杏仁核到颞下回TE区和枕叶皮质VI区的投射。前皮质区域参与面部处理，已知从杏仁核接收强大的投射。在第二阶段的研究中，将用抗细小病毒抗体对组织进行双重标记