Projections from the amygdala to the cortex: an EM study

这个项目的目的是描述，在电子显微镜水平，杏仁核和视觉新皮层之间的连接非人灵长类动物。杏仁核对情绪反应至关重要，它可能与包括癫痫和自闭症在内的多种疾病有关。虽然人们知道杏仁核和大脑皮层紧密相连，但对这些连接的突触组织却知之甚少。顺行示踪剂Phaseolus vulgarie-白细胞凝集素（PHA-L）将立体定向注射到成年雄性恒河猴的杏仁核中。透射示踪剂将在电子显微镜下表征从杏仁核到颞下回TE区和枕叶皮层VI区的投射。前皮层区域参与面部处理，并接受来自杏仁核的强烈投射。在研究的第二阶段，组织将被双重标记为parva抗体

The objective of this project is to describe, at the electron microscopic level ,the connection between the amygdala and the visual neocortex of non-human primates. The amygdala is essential for emotional response, and it may be involved in a variety of illnesses, including epilepsy and autism. While it known that the amygdala and the cortex are heavily interconnected, little is known concerning the synaptic organization of these connections. The anterograde tracer, Phaseolus vulgarie- leucoagglutinin (PHA-L), will be stereotaxically injected into the amygdala of adult male rhesus monkeys. The transported tracer will under the electron microscope to characterize the projections from the amygdala to area TE in the inferior temporal gyrus and VI in the occipital cortex. The former cortical areas is involved in facial processing and is known to receive strong projections from the amygdala. In second phase of the study, tissue will be double-labeled with an antibody to parva