Proteins Surface Characterisation

蛋白质是许多工业过程的关键部分。它们在诊断和治疗人类疾病方面也变得越来越重要。它们是高度可变、敏感和多变的生物大分子。它们的复杂性对其使用构成了智力上的挑战。这项研究工作的一个关键宗旨是了解蛋白质在自然界中的行为。生物系统已经进化了数百万年。在生物科学和工程中遇到的许多研究问题已经被进化和自然选择多次解决。例如，蛋白质在细胞环境中自然聚集。存在控制这一点的系统。当这些系统失效时，人类疾病，如阿尔茨海默氏症就会发展。蛋白质聚集已经被自然界所理解和掌握。这个项目提出了一种新的方法来表征蛋白质的表面。它使用现有的高压液相色谱（HPLC）技术，是对先前在自相互作用色谱（SIC）中进行的研究的发展-亲和色谱的一种形式。蛋白质表面表征色谱（ProChrom）旨在为科学家和工程师提供另一种工具来了解实验室和自然生化环境。色谱法本质上是一种状态的直接热力学测量。这可以为研究困难的生物环境提供独特的视角。ProChrom的目的是补充，而不是必须取代，已建立的表征技术。如果成功实施，它可以形成一个全新的科学研究领域的基础。

Proteins are a key part of many industrial processes. They are also becoming increasingly important in the diagnosis and treatment of human disease. They are highly variable, sensitive and changeable biological macromolecules. Their sheer complexity is an intellectual challenge to their use.A key ethos of this research effort is to learn how proteins behave in nature. Biological systems have evolved over millions of years. Many of the research problems that are encountered in bioscience and engineering have been solved many times over by evolution and natural selection. For example, proteins naturally aggregate in cellular environments. There exist systems to control this. When these systems fail, human disease, such as Alzheimer's can develop. Protein aggregation has been understood and mastered by nature.This project proposes a novel approach to the characterisation of the surfaces of proteins. It uses existing High Pressure Liquid Chromatography (HPLC) technologies and is a development of previous research carried out in Self Interaction Chromatography (SIC) - a form of Affinity Chromatography. Protein Surface Characterisation Chromatography (ProChrom) aims to provide scientists and engineers with another tool to understand laboratory and natural biochemical environments. Chromatography is inherently a direct thermodynamic measurement of state. This could provide a unique insight into difficult biological situations. The aim of ProChrom is to complement, rather than necessarily replace, established characterisation techniques. If successfully implemented, it could form the basis for an entirely new field of scientific research.