XRAY DIFFRACTION ANALYSES OF PLANT VIRUSES

植物病毒的 X 射线衍射分析

• 批准号: 6437454
• 负责人: MARY A CANADY
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6437454
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology; virus

We have used synchrotron radiation for high resolution structural studies of plant viruses. Diffraction data were collected from crystals of Satellite Panicum Mosaic Virus (SPMV) leading to a 1.9 E resolution structure of the T=1 virus. Diffraction data from crystals of Turnip Yellow Mosaic Virus (TYMV), a T=3 virus with a very large unit cell, were also collected to 3.5 E resolution. These data, which are unattainable using conventional X-ray generators, are being used in the structural determination of TYMV. Plant virus structural studies are important in understanding virus structure, but also provide a convenient way to study the ways in which X-ray data collection and data processing of large unit cells can be done successfully.

我们已经使用同步辐射进行高分辨率结构 植物病毒的研究 衍射数据收集自 卫星黍花叶病毒（SPMV）的晶体，导致1.9 E T=1病毒的解析结构。 晶体衍射数据 芜菁黄花叶病毒（TYMV），一种T=3病毒，具有非常大的 单位细胞，也收集到3.5 E分辨率。 这些数据， 使用传统的X射线发生器是无法实现的， TYMV的结构测定。 植物病毒结构 研究对于理解病毒结构很重要，但也 提供了一种方便的方法来研究X射线数据 可以完成大单位单元的收集和数据处理 成功地