XRAY DIFFRACTION ANALYSES OF PLANT VIRUSES

我们已经使用同步辐射来实现高分辨率的结构 对植物病毒的研究。衍射数据收集自 卫星Panicum花叶病毒(SPMV)的晶体导致1.9E T=1病毒的解析结构。晶体的衍射数据 芜菁黄花叶病毒(TYMV)是一种T=3病毒，具有很大的 单位细胞，也收集到3.5E分辨率。这些数据，即 是用传统的X射线发生器无法达到的，正在使用 在TYMV的结构测定中。植物病毒结构 研究对了解病毒结构很重要，但也 提供了一种方便的方法来研究X射线数据 可以进行大型单元格的采集和数据处理 成功了。

We have used synchrotron radiation for high resolution structural studies of plant viruses. Diffraction data were collected from crystals of Satellite Panicum Mosaic Virus (SPMV) leading to a 1.9 E resolution structure of the T=1 virus. Diffraction data from crystals of Turnip Yellow Mosaic Virus (TYMV), a T=3 virus with a very large unit cell, were also collected to 3.5 E resolution. These data, which are unattainable using conventional X-ray generators, are being used in the structural determination of TYMV. Plant virus structural studies are important in understanding virus structure, but also provide a convenient way to study the ways in which X-ray data collection and data processing of large unit cells can be done successfully.