Assessment of the potential hazards of 2D nanomaterials to the environment

该项目目前的重点是合成一组高度控制横向尺寸的氧化石墨烯2dnm模型，并测试2dnm的横向尺寸如何改变土壤中三种不同细菌的生存能力（包括细胞代谢和膜完整性）。还将评估先前与天然有机物相互作用对细菌随后毒性的影响。毒理学终点将与高分辨率电子显微镜研究结果相结合，以将细胞内/细胞外氧化石墨烯的位置与细胞器的细胞代谢改变以及细胞反应性联系起来。由于氧化石墨烯与细菌相互作用的成像存在成像挑战，鉴于其与细胞的低对比度，我还将评估二硫化钼与细菌的相互作用。

The current focus of this project is to synthesise a set of model GO 2DNMs with highly controlled lateral size and test how the lateral dimension of the 2DNMs alters the viability (including cell metabolism and membrane integrity) of three different bacteria found in soils. The effect of prior interactions with natural organic matter on their subsequent toxicity of the bacteria will also be assessed. Toxicological endpoints will be coupled to the results of high resolution electron microscopy studies to relate intra/extracellular GO location to altered cellular metabolism of cell organelles and thus cellular reactivity. Since imaging the interaction of GO with bacteria presents an imaging challenge, given its low contrast against the cells, I will also assess the interaction of molybdenum disulphide with bacteria.