STP & TIP ARE ESSENTIAL FOR HERPESVIRUS SAIMIRI ONCOGENICITY

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• 批准号: 6453742
• 负责人: Samuel Monroe DUBOISE
• 金额: $ 11.11万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6453742
• 关键词: Mammalia; animal tissue; communicable diseases; virus

Mutant forms of herpesvirus saimiri (HVS) subgroup C strain 488 with deletions in either STP-C488 or Tip were constructed The transforming potentials of the HVS mutants were tested in cell culture and in common marmosets Parental "VS subgroup C strain 488 immortalized common marmoset T lymphocytes in vitro to interleukin-2-independent growth, but neither of the deletion mutants produced such growth transformation Wild-type "VS produced fatal lymphoma within 19 to 20 days of experimental infection of common marmosets, while "VS ASTP-C488 and "VS ATip were nononcogenic Virus was repeatedly isolated from the peripheral blood of marmosets infected with mutant virus for more than 5 months These results demonstrate that STP-C488 and Tip are not required for replication or persistence, but each is essential for transformation in cell culture and for lymphoma induction in common marmosets PUBLICATIONS Duboise, SM, Guo, J, Czajak, S, Desrosiers, RC and Jung, JU STP and Tip are Essential for Herpesvirus Saimiri Oncogenicity J Virol 1998; 72:1308-1313

疱疹病毒 saimiri (HVS) 亚组 C 株 488 的突变形式 构建了 STP-C488 或 Tip 中的缺失 在细胞培养物中测试了 HVS 突变体的转化潜力 和普通狨猴亲本“VS 亚组 C 品系 488 永生化普通狨猴 T 淋巴细胞 不依赖白细胞介素 2 的生长，但两个缺失突变体都没有 产生了这样的生长转变“野生型”VS产生了致命的 常见实验性感染后 19 至 20 天内发生淋巴瘤 狨猴，而“VS ASTP-C488”和“VS ATip”是非致癌病毒 多次从狨猴的外周血中分离出来 感染变异病毒超过5个月这些结果 证明复制不需要 STP-C488 和 Tip 或 持久性，但每一个对于细胞培养中的转化都是必不可少的 以及普通狨猴的淋巴瘤诱导出版物 Duboise， SM、Guo、J、Czajak、S、Desrosiers、RC 和 Jung、JU STP 和 Tip 是 疱疹病毒必不可少 Saimiri 致癌性 J Virol 1998； 72:1308-1313