STP & TIP ARE ESSENTIAL FOR HERPESVIRUS SAIMIRI ONCOGENICITY

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• 批准号: 6591296
• 负责人: Samuel Monroe DUBOISE
• 金额: $ 11.11万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6591296
• 关键词: Mammalia; animal tissue; communicable diseases; virus

Mutant forms of herpesvirus saimiri (HVS) subgroup C strain 488 with deletions in either STP-C488 or Tip were constructed The transforming potentials of the HVS mutants were tested in cell culture and in common marmosets Parental "VS subgroup C strain 488 immortalized common marmoset T lymphocytes in vitro to interleukin-2-independent growth, but neither of the deletion mutants produced such growth transformation Wild-type "VS produced fatal lymphoma within 19 to 20 days of experimental infection of common marmosets, while "VS ASTP-C488 and "VS ATip were nononcogenic Virus was repeatedly isolated from the peripheral blood of marmosets infected with mutant virus for more than 5 months These results demonstrate that STP-C488 and Tip are not required for replication or persistence, but each is essential for transformation in cell culture and for lymphoma induction in common marmosets PUBLICATIONS Duboise, SM, Guo, J, Czajak, S, Desrosiers, RC and Jung, JU STP and Tip are Essential for Herpesvirus Saimiri Oncogenicity J Virol 1998; 72:1308-1313

松鼠猴疱疹病毒C亚群488株的突变型 构建了STP-C488或Tip缺失的细胞。 在细胞培养中测试HVS突变体的转化潜力 在普通绒猴亲本“VS C亚群488株中 体外永生化普通绒猴T淋巴细胞， 白细胞介素-2非依赖性生长，但缺失突变体 野生型“VS产生了致命的 实验性感染后19至20天内的淋巴瘤 绒猴，而“VS ASTP-C488和“VS ATip是非致癌病毒 从绒猴的外周血中反复分离 感染变异病毒5个月以上 证明复制不需要STP-C488和头端，或 持久性，但每一个都是细胞培养转化所必需的 以及普通绒猴中的淋巴瘤诱导 SM，Guo，J，Czajak，S，Desrosiers，RC和Jung，JU STP和Tip是 疱疹病毒Saimiri的致瘤性J Virol 1998; 72：1308-1313