ROLE FOR HERPESVIRUS SAIMIRI ORF14 IN TRANSFORMATION & PERSISTENT INFECTION

疱疹病毒开放阅读框架14(Orft4)的产物 Saimiri(HVS)与小鼠乳腺肿瘤有显著同源性 病毒超抗原orfl4编码一个50 kDa的分泌型糖蛋白，如 之前展示了(Z姚，E Maraskovsky，M K Spriggs，J I Cohen，R J 《免疫学杂志》156：3260-3266,1996)或4 重组杆状病毒的表达可有效诱导 几种不同来源的CD4阳性细胞的增殖 研究orfl4在转化中的作用的物种，orfl4是 HVS(HVS Orfl4)是通过orfl4基因缺失而构建的 在细胞培养和细胞培养中检测了hVS orfl4的转化潜能。 恒河猴父母C亚群488株恒河猴永生 常见绒猴T淋巴细胞对白细胞介素2非依赖性的体外研究 生长，而HVS orft4突变体不能产生这样的生长 此外，hvs或fl4通常是非致癌的。 与其他非致癌的人类免疫缺陷病毒突变病毒相比， 从小鼠外周血单核细胞中反复分离 感染5个月以上的绒猴，人类免疫缺陷病毒或流感病毒没有持续存在 在体内的高水平上，这些结果表明人类免疫缺陷病毒的orfl4是 复制不需要，但转换和 对于体内高水平持久性出版物Duboise，SM，Guo，J， 沙加，S，李，H，维兹，德罗西尔斯，RC和荣格，Ju A角色 疱疹病毒Simiri orf14转化和持续感染J Virol 1998；72：6770-6776

The product of open reading frame 14 (orft4) of herpesvirus saimiri (HVS) exhibits significant homology with mouse mammary tumor virus superantigen orfl4 encodes a 50-kDa secreted glycoprotein, as shown previously (Z Yao, E Maraskovsky, M K Spriggs, J I Cohen, R J Armitage, and M R Alderson, J Immunol 156:3260-3266, 1996) orfl4 expressed from recombinant baculovirus powerfully induces proliferation of CD4-positive cells originating from several different species To study the role of orfl4 in transformation, a mutant form of HVS (HVS orfl4) was constructed with a deletion in the orfl4 gene The transforming potential of HVS orfl4 was tested in cell culture and in common marmosets Parental HVS subgroup C strain 488 immortalized common marmoset T lymphocytes in vitro to interleukin-2-independent growth, while the HVS orft4 mutant did not produce such a growth transformation In addition, HVS orfl4 was nononcogenic in common marmosets In contrast to oth er nononcogenic HVS mutant viruses which were repeatedly isolated from peripheral blood mononuclear cells of infected marmosets for more than 5 months, HVS orfl4 did not persist at a high level in vivo These results demonstrate that orfl4 of HVS is not required for replication but is required for transformation and for high-level persistence in vivo PUBLICATIONS Duboise, SM, Guo, J, Czajak, S, Lee, H, Veazey, Desrosiers, RC and Jung, JU A role for herpesvirus saimiri orf14 in transformation and persistent infection J Virol 1998; 72:6770-6776