Core--3D imaging

成像核心是围绕最先进的设施、光学和电子显微镜人员的专业知识以及每个子项目调查人员在成像方面的经验而建立的。中央核心设施将构成该计划的互动重点，并将提供一致的成像性能和解释。该设备配备了定量共焦和双光子显微镜(Bio-Rad MRC-1024)、数字去卷积广域显微镜(应用Precision Deltavision去卷积软件)和电子显微镜(Phillips CM120 TEM)。成像核心设备的目标是应用、开发和结合这些技术，为极化上皮细胞的三维显微成像提供一种合理、综合的方法。核心设施的具体任务将是，1.提供定量成像，以支持4个计划子项目中的每一个；2.实施数字图像去卷积技术，以支持使用共焦显微镜、双光子显微镜以及王尔德视野和共焦图像的图像去卷积的高分辨率三维光学成像的4路策略；以及3.开发活的极化上皮细胞的三维成像方法。随着这些目标的完成，成像核心将发展成为研究肾细胞生物学的独特资源。

The imaging core is build around a state-of-the-art facility, the expertise of the optical and electron microscopy personnel and the experience of each of the subproject investigators in imaging. The central core facility will constitute a focus of interaction for the program, and will provide for consistent imaging performance and interpretation. The facility is equipped for quantitative confocal and 2-photon microscopy (Bio-Rad MRC-1024), digital deconvolution wide-field microscopy (Applied Precision Deltavision deconvolution software) and electron microscopy (Phillips CM120 TEM). It is the objective of the imaging core facility to apply, develop and combined these technologies to provide a rational, integrated approach to three-dimensional microscopic imaging of polarized epithelial cells. The specific mission of the core facility will be to, 1. provide quantitative imaging to support each of the 4 program subprojects, 2. implement digital image deconvolution techniques to support a 4 way strategy of high resolution three-dimensional optical imaging employing confocal microscopy, 2-photon microscopy and image deconvolution of wilde-field and confocal images and, 3. develop methods of three-dimensional imaging of living polarized epithelial cells. With the completion of these aims, the imaging core will have been developed into a unique resource for the study of renal cell biology.