F0F1 ATPASE STRUCTURAL STUDIES

F0F1 ATP酶结构研究

• 批准号: 6504527
• 负责人: PETER L PEDERSEN
• 金额: $ 13.47万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-12-01 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6504527
• 关键词: animal tissue; biomedical resource; enzymes; human tissue; respiratory system

The ATP synthase from mitochondria of animal cells is an important enzyme which couples proton translocation to ATP synthesis, to provide chemical energy needed for many biological processes. It consists of F1 (a soluble domain with 5 subunit types of a total mass of 370 kD), a stalk and F0 (a membrane domain with at least three subunit types). The x ray crystallographic structure for the F1 has been determined to 2.8
but the structure of the rest of the molecule is still unknown. We have carried out preliminary experiments to image the entire enzyme with the hope that we can define the overall organization of the three components of this large enzyme.

来自动物细胞线粒体的ATP合酶是一种重要的 一种将质子转运与ATP合成偶联的酶， 许多生物过程所需的化学能。 它包括 F1（总质量为370 kD的具有5种亚基类型的可溶性结构域）， 茎和F0（具有至少三种亚基类型的膜结构域）。 F1的X射线晶体结构已被确定， 2.8
但分子其余部分的结构仍然未知。 我们已经进行了初步实验， 希望我们能够定义这三个组织的整体结构， 这种酶的主要成分。