F0F1 ATPASE STRUCTURAL STUDIES

F0F1 ATP酶结构研究

• 批准号: 6504527
• 负责人: PETER L PEDERSEN
• 金额: $ 13.47万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-12-01 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6504527
• 关键词: animal tissue; biomedical resource; enzymes; human tissue; respiratory system

The ATP synthase from mitochondria of animal cells is an important enzyme which couples proton translocation to ATP synthesis, to provide chemical energy needed for many biological processes. It consists of F1 (a soluble domain with 5 subunit types of a total mass of 370 kD), a stalk and F0 (a membrane domain with at least three subunit types). The x ray crystallographic structure for the F1 has been determined to 2.8
but the structure of the rest of the molecule is still unknown. We have carried out preliminary experiments to image the entire enzyme with the hope that we can define the overall organization of the three components of this large enzyme.

来自动物细胞线粒体的ATP合酶是重要的 将质子易位到ATP合成的酶，以提供 许多生物过程所需的化学能。 它由 F1（一个可溶域，具有5个亚基类型的总质量为370 kd）， 茎和F0（具有至少三种亚基类型的膜结构域）。 F1的X射线晶体学结构已确定为 2.8但是分子其余的结构仍然未知。 我们已经进行了初步实验，以对整个酶进行成像 希望我们可以定义三个的整体组织 这种大酶的成分。