CARIBBEAN OCTOCORAL--SOURCE FOR ANTITUBERCULAR COMPOUNDS

加勒比八珊瑚——抗结核化合物的来源

• 批准号: 6472788
• 负责人: ABIMAEL D RODRIGUEZ
• 金额: $ 11.03万
• 依托单位: UNIVERSITY OF PUERTO RICO RIO PIEDRAS
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6472788
• 关键词: Caribbean islands; X ray crystallography; animal extract; antitubercular agents; aquatic organism; biological products; chemical structure function; coral; drug discovery /isolation; drug screening /evaluation; field study; gas chromatography; infrared spectrometry; laboratory mouse; liquid chromatography; mass spectrometry; nuclear magnetic resonance spectroscopy; saltwater environment; ultraviolet spectrometry

Description (Adapted from Application): Marine invertebrates have been identified for a long time by chemists as likely sources of new biomedicinal substances. Pharmacologists, physiologists, and biochemists have demonstrated that many of these novel marine products modify fundamental life processes in ways suggesting biomedical applications. These molecules can serve as leads to guide the pharmaceutical and chemical industries in developing new products. This research aims specifically at Caribbean gorgonian octocorals as a likely resource for novel anti-tuberculosis natural products. Since less than 20 percent of the species of gorgonian octocorals known to exist in the Caribbean region have been assessed chemically for their secondary metabolite composition, and many of these have been shown to produce metabolites possessing potent chemotherapeutic properties, gorgonians from this region have the potential to contain a wealth of novel drugs. Specifically, during the four years of this research, the investigators plan to carefully scrutinize the pharmacology and chemistry of 40 or so representative species belonging to all the major Caribbean gorgonian genera. Initial extraction with solvents of different polarities (e.g., hexane, methylene chloride, and methanol) will be monitored using anti-tuberculosis activity assays. Only gorgonian extract residues possessing strong anti-tuberculosis properties will be screened further for chemical structural features by 1H- and 13C-NMR spectroscopy. Further purification by chromatography (column, GC, LC) and determination of molecular structure will proceed if warranted by the screening results. Structure elucidation will rely heavily on spectral methods (e.g., 1H- and 13C-NMR, MS, IR, UV) and X-ray diffraction techniques.

描述(改编自应用程序)：海洋无脊椎动物 长期以来被化学家确定为新生物医学的可能来源 物质。药理学家、生理学家和生化学家已经证明 其中许多新的海洋产品改变了基本的生命过程 建议生物医学应用的方法。这些分子可以作为线索 引导医药化工开发新产品。 这项研究专门针对加勒比海柳珊瑚，认为这可能是 新的抗结核病天然产品的资源。因为不到20 加勒比海已知的柳珊瑚种类的百分比 已对该区域的次生代谢物进行了化学评估 成分，其中许多已被证明能产生代谢物 具有强大的化疗特性，来自该地区的柳珊瑚 蕴藏着丰富新药的潜力。具体地说，在四个 经过多年的研究，调查人员计划仔细审查 All属约40种代表性物种的药理和化学成分 加勒比海柳珊瑚的主要属。用乙酸乙酯的溶剂进行初步提取 不同的极性(例如，己烷、二氯甲烷和甲醇)将是 使用抗结核活性分析进行监测。只有柳珊瑚提取物 具有较强抗结核病特性的残留物将被筛选 通过~1H-和~(13)C-核磁共振波谱进一步研究其化学结构特征。 柱层析(柱层析、GC、LC)进一步纯化及测定 如果筛选结果证实，将继续进行分子结构。 结构鉴定将在很大程度上依赖于光谱方法(例如，1H-和 ~(13)C-核磁共振、MS、IR、UV)和X射线衍射技术。