CARIBBEAN OCTOCORAL--SOURCE FOR ANTITUBERCULAR COMPOUNDS

加勒比八珊瑚——抗结核化合物的来源

• 批准号: 6472788
• 负责人: ABIMAEL D RODRIGUEZ
• 金额: $ 11.03万
• 依托单位: UNIVERSITY OF PUERTO RICO RIO PIEDRAS
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6472788
• 关键词: Caribbean islands; X ray crystallography; animal extract; antitubercular agents; aquatic organism; biological products; chemical structure function; coral; drug discovery /isolation; drug screening /evaluation; field study; gas chromatography; infrared spectrometry; laboratory mouse; liquid chromatography; mass spectrometry; nuclear magnetic resonance spectroscopy; saltwater environment; ultraviolet spectrometry

Description (Adapted from Application): Marine invertebrates have been identified for a long time by chemists as likely sources of new biomedicinal substances. Pharmacologists, physiologists, and biochemists have demonstrated that many of these novel marine products modify fundamental life processes in ways suggesting biomedical applications. These molecules can serve as leads to guide the pharmaceutical and chemical industries in developing new products. This research aims specifically at Caribbean gorgonian octocorals as a likely resource for novel anti-tuberculosis natural products. Since less than 20 percent of the species of gorgonian octocorals known to exist in the Caribbean region have been assessed chemically for their secondary metabolite composition, and many of these have been shown to produce metabolites possessing potent chemotherapeutic properties, gorgonians from this region have the potential to contain a wealth of novel drugs. Specifically, during the four years of this research, the investigators plan to carefully scrutinize the pharmacology and chemistry of 40 or so representative species belonging to all the major Caribbean gorgonian genera. Initial extraction with solvents of different polarities (e.g., hexane, methylene chloride, and methanol) will be monitored using anti-tuberculosis activity assays. Only gorgonian extract residues possessing strong anti-tuberculosis properties will be screened further for chemical structural features by 1H- and 13C-NMR spectroscopy. Further purification by chromatography (column, GC, LC) and determination of molecular structure will proceed if warranted by the screening results. Structure elucidation will rely heavily on spectral methods (e.g., 1H- and 13C-NMR, MS, IR, UV) and X-ray diffraction techniques.

描述（改编自申请）：海洋无脊椎动物已被 长期以来被化学家认为是新生物医学的可能来源 物质。药理学家、生理学家和生物化学家已经证明 许多这些新颖的海洋产品改变了基本的生命过程 建议生物医学应用的方法。这些分子可以作为线索 指导医药、化工行业新产品开发。 这项研究特别针对加勒比柳珊瑚八珊瑚作为可能的 新型抗结核天然产物资源。由于不到20 已知存在于加勒比地区的柳珊瑚八珊瑚物种的百分比 该区域已对其次生代谢物进行了化学评估 成分，其中许多已被证明可以产生代谢物 来自该地区的柳珊瑚具有有效的化疗特性 蕴含大量新型药物的潜力。具体而言，四 经过多年的研究，研究人员计划仔细审查 属于全部的 40 多个代表性物种的药理和化学 主要的加勒比柳珊瑚属。初始萃取溶剂为 不同极性（例如己烷、二氯甲烷和甲醇）将 使用抗结核活性测定进行监测。仅柳珊瑚提取物 将筛选具有强抗结核特性的残留物 通过 1H 和 13C-NMR 光谱进一步了解化学结构特征。 通过色谱法（柱、GC、LC）进一步纯化并测定 如果筛选结果有保证，分子结构将继续进行。 结构解析将在很大程度上依赖于光谱方法（例如 1H- 和 13C-NMR、MS、IR、UV）和 X 射线衍射技术。