CORE--FLOW CYTOMETRY FACILITY

核心——流式细胞仪

• 批准号: 6470585
• 负责人: DORIS B TSE
• 金额: $ 17.11万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6470585
• 关键词: AIDS; HIV infections; biomedical facility; clinical research; flow cytometry; human immunodeficiency virus; human subject

The CFAR Flow Cytometry is heavily used by investigators in all programs of the Center. The core provides education and consultations with CFAR members concerning the potential uses of this powerful method of analysis, and the appropriate design of experiments. Core provides an array of innovative measurements in addition to the usual 3- color surface phenotyping of lymphocytes. Our instrumentation consists of 2 FACSCAN and 2 Profile analytical flow cytometers, each equipped for three-color detection, a Becton Dickinson (BD) FACSCalibur and a B.D. Vantage dual laser sorting instrument with a cloning deposition device. This instrument is located in a negative air pressure room with BL-3 category air filtration and HEPA filtration of all air leaving the instrument. This enable us to sort infected cells. Charge back fees to scientists using the facility provide partial recovery of costs.

CFAR流式细胞术在中心的所有项目中被研究者大量使用。核心提供教育和咨询与CFAR成员关于这种强大的分析方法的潜在用途，并适当设计的实验。核心提供了一系列创新的测量除了通常的3色表面表型的淋巴细胞。我们的仪器包括2台FACSCAN和2台Profile分析流式细胞仪，每台都配备了三色检测，一台Becton Dickinson（BD）FACSCalibur和一台B.D.带有克隆沉积装置的Vantage双激光分选仪。该仪器位于负压室中，对所有离开仪器的空气进行BL-3级空气过滤和HEPA过滤。这使我们能够对受感染的细胞进行分类。向使用该设施的科学家收取费用可收回部分成本。