FLOW CYTOMETRY CORE

核心D：描述(申请人提供)：CFAR流式细胞仪核心被大量使用 由中心所有项目的调查人员提供。该中心通过提供有关这一强大技术的教育和咨询，促进了流式细胞术在艾滋病毒研究中的应用 研究人员对研究项目的规划和拟议实验的设计进行了指导。这个 CORE在临床测试中获得了四色淋巴细胞免疫表型的国家认证，并已 在其研究中积累了广泛的传统和创新方法 曲目。我们的仪器包括两台用于三色检测的FACScans，两台用于四色检测的FACSCaliburr，以及一台位于BL-3级实验室的FACSVantage SE。它配备了三个用于七色检测的激光，一个生物危害遏制单元，一个细胞沉积单元，以及一个等温再循环器，用于对暴露在艾滋病毒和其他传染性微生物中的细胞进行分类和克隆。向使用这一设施的科学家收取的费用可收回拟议预算中未涵盖的费用。

CORE D: DESCRIPTION (provided by applicant): The CFAR Flow Cytometry Core is heavily used by investigators in all programs of the Center. The core promotes application of flow cytometry in HIV research by providing education on this powerful technology and consultation with investigators on the planning of research projects and the design of proposed experiments. The core is state-certified for 4-color lymphocyte immunophenotyping in clinical testing and has accrued an extensive array of conventional and innovative methodologies in its research repertoire. Our instrumentation consists of 2 FACScans for 3-color detection, 2 FACSCaliburs for 4-color detection, and a FACSVantage SE located in a BL-3 level laboratory. It is equipped with 3 lasers for 7-color detection, a biohazard containment unit, a cell deposition unit, and an isotemp recirculator for the sorting and cloning of cells exposed to HIV and other infectious microbes. Charge-back fees to scientists using this facility provide recovery of costs not covered in the proposed budget.