FLOW CYTOMETRY CORE

核心D：描述（由申请人提供）：CFAR流式细胞术核心被大量使用 研究人员在该中心的所有项目。该中心通过提供关于流式细胞术这一强大技术的教育和咨询， 研究人员对研究项目的规划和拟议实验的设计。的 核心是国家认证的四色淋巴细胞免疫表型在临床测试，并已 在其研究中积累了广泛的传统和创新方法 保留曲目。我们的仪器包括2个用于3色检测的FACScans、2个用于4色检测的FACSCalibur和位于BL-3级实验室的FACSVantage SE。它配备了3个用于7色检测的激光器，一个生物危害控制单元，一个细胞沉积单元和一个用于分选和克隆暴露于HIV和其他传染性微生物的细胞的isotemp再循环器。向使用这一设施的科学家收取费用，以收回拟议预算中未包括的费用。

CORE D: DESCRIPTION (provided by applicant): The CFAR Flow Cytometry Core is heavily used by investigators in all programs of the Center. The core promotes application of flow cytometry in HIV research by providing education on this powerful technology and consultation with investigators on the planning of research projects and the design of proposed experiments. The core is state-certified for 4-color lymphocyte immunophenotyping in clinical testing and has accrued an extensive array of conventional and innovative methodologies in its research repertoire. Our instrumentation consists of 2 FACScans for 3-color detection, 2 FACSCaliburs for 4-color detection, and a FACSVantage SE located in a BL-3 level laboratory. It is equipped with 3 lasers for 7-color detection, a biohazard containment unit, a cell deposition unit, and an isotemp recirculator for the sorting and cloning of cells exposed to HIV and other infectious microbes. Charge-back fees to scientists using this facility provide recovery of costs not covered in the proposed budget.