REGENERATION OF CD4 LYMPHOCYTES

CD4 淋巴细胞的再生

• 批准号: 2673122
• 负责人: DORIS B TSE
• 金额: $ 8.38万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2673122
• 关键词: AIDS therapy; CD4 molecule; DNA; HIV infections; antiviral agents; apoptosis; biomarker; cell adhesion molecules; cell cycle; cell proliferation; clinical research; cyclins; cytokine; cytokine receptors; flow cytometry; helper T lymphocyte; human subject; receptor expression; virus load

DESCRIPTION (adapted from applicant's abstract): The primary aim of this project is to determine if (i) apoptosis of the cycling pool, or (ii) cell cycle arrest is contributing to the failure of CD4 cells to regenerate, and (iii) identify the potential cellular lesions associated therewith. Towards this goal, the investigators will focus on longitudinal samples obtained during the acute phase of anti-viral therapy. They will determine by FACS analysis the presence or intensity of cytokine receptors, adhesion molecules, apoptotic markers, DNA content, and restriction point-related cyclins on Ki67-expressing CD4 cells and their ex vivo cytokine response. By comparing the data obtained at time points which display different kinetics of CD4 lymphocyte regeneration, they hope to better define the conditions which potentiate or inhibit peripheral CD4 expansion.

描述（改编自申请人的摘要）：本报告的主要目的 项目是确定（i）循环池的凋亡，或（ii）细胞 周期停滞导致 CD4 细胞无法再生，并且 (iii)识别与其相关的潜在细胞损伤。 向 为了实现这一目标，研究人员将重点关注获得的纵向样本 在抗病毒治疗的急性期。 他们将通过FACS确定 分析细胞因子受体的存在或强度、粘附 分子、凋亡标记、DNA 含量和限制点相关 表达 Ki67 的 CD4 细胞上的细胞周期蛋白及其离体细胞因子反应。 通过比较显示不同时间点获得的数据 CD4淋巴细胞再生动力学，他们希望更好地定义 增强或抑制外周 CD4 扩增的条件。

项目成果

Heterogeneity in fetal immunocompetence during the second trimester of gestation. Implications for treatment of nonimmune genetic disorders by in utero transplantation.

妊娠中期胎儿免疫能力的异质性。

• DOI: 10.1159/000083900
• 发表时间: 2005
• 期刊: Fetal diagnosis and therapy.
• 作者: Tse,DorisB;Ching,Elbert;Yousefzadeh,Nora;Roque,Hank;Young,BruceK
• 通讯作者: Young,BruceK