FACSAria Flow Cytometer

描述（由申请人提供）：在过去的20年里，流式细胞术已经发展成为一种技术，可以检测细胞表面的分子，监测从基因调控到蛋白质-蛋白质相互作用的细胞过程，以及从细菌到哺乳动物的细胞的快速分离，显示理想的表型。直到最近三年，能够进行多色检测和高速细胞分离的荧光激活细胞分选器，如我们的facadvantage（1994年在NIH的支持下获得），都是大型复杂的仪器，需要特殊的实验室设施和高成本维护。FACSAria是新一代台式细胞分选机，在光学、检测灵敏度、流体、采集速率和分选能力方面取得了重大进展。它是我们facadvantage的及时和重要的替代品。两个主要用户的到来，以及最近成立的医学寄生虫学部门，为纽约医科大学的流式细胞术应用带来了前所未有的需求。FACSAria将提供高速细胞分离、特殊的病原体控制、额外的颜色检测、最大的操作效率和低维护成本。NYUSM没有类似的仪器。在CFAR流式细胞术核心项目的支持下，该项目将成为纽约大学及其附属机构和邻近研究机构的所有科学家共享的资源。使用情况将由CFAR执行委员会与内部和外部咨询委员会进行监控。操作员工资、耗材和维护费将部分由NIH奖励（P30 AI27742）支付，部分由正在进行的用户退款系统下的个人补助金支付。谢博士拥有丰富的流式细胞术实践经验，将促进仪器的使用和创新研究应用，验证数据解释，监督操作人员，并要求质量控制。该仪器将用于研究感染病原体的人类细胞和动物细胞。这些研究将促进我们对许多疾病的病因和病理的理解，包括艾滋病毒、结核病、哮喘、败血症、疟疾、肝炎、睡眠呼吸暂停和环境毒物暴露。这些信息将有助于发展或改进预防和治疗这些疾病的医疗程序。

DESCRIPTION (provided by applicant): Over the last 2 decades, flow cytometry has evolved into a technology that provides for the detection of molecules on the cell surface, the monitoring of cellular processes from gene regulation to protein-protein interaction, and the rapid isolation of cells, from bacterial to mammalian, that display desirable phenotypes. Until the last three years, fluorescence-activated cell sorters capable of multicolor detection and high-speed cell isolation, such as our FACSVantage (acquired in 1994 with NIH support), are large intricate instruments that require special laboratory facilities and high cost maintenance. The FACSAria is a new generation bench-top cell sorter with significant advances in optics, detection sensitivity, fluidics, acquisition rate, and sort capability. It is a timely and vital replacement for our FACSVantage. The arrival of two major users, and a recently established Department of Medical Parasitology, bring unprecedented demands on flow cytometry utilization at NYUSM. The FACSAria will provide high speed cell isolation, exceptional pathogen containment, additional color detection, maximal operator productivity, and low maintenance costs. There is no comparable instrument at NYUSM. Under the auspices of the CFAR Flow Cytometry Core directed by Dr. Doris Tse, it will be a shared resource available to all scientists at NYUSM, its affiliates, and neighboring research institutions. Usage will be monitored by the CFAR executive committee with an internal and external advisory board. Operator salary, consumable supplies, and maintenance fees will be covered in part by an NIH award (P30 AI27742), and charged in part to individual grants under an ongoing user-chargeback system. Dr. Tse, who has extensive hands-on flow cytometry experience, will promote instrument utilization and innovative research applications, validate data interpretation, supervise operators, and mandate quality controls. This instrument will be used to study human cells and animal cells infected with pathogens. These studies will advance our understanding of the etiology and pathology underlying many diseases, including HIV, TB, asthma, sepsis, malaria, hepatitis, sleep apnea, and environmental toxicant exposure. This information will potentiate the development or improvement of medical procedures towards their prevention and treatment.