Microstructural Dynamics of Reticulocyte Membranes

网织红细胞膜的微观结构动力学

• 批准号: 6492644
• 负责人: JAMES C LEE
• 金额: $ 4.42万
• 依托单位: U.S. DEPARTMENT OF ENERGY-LBNL
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-30 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6492644
• 关键词: cell biology; cell membrane; cytoskeletal proteins; flow cytometry; membrane activity; membrane proteins; postdoctoral investigator; protein protein interaction; reticulocytes; western blottings

At the conclusion of erythropoiesis, erythroblasts expel their nuclei to become reticulocytes. In this process, partitioning of plasma membrane components between the extruded nucleus and young reticulocyte is governed by the molecular organization of the membrane and skeletal network. The differences in morphology and mechanical properties of reticulocytes and erythrocytes suggest alterations of membrane organization during reticulocyte maturation. Such organizational changes may also cause the loss of function of adhesion molecules, such as Lu, LW and CD47, in erythrocytes. Little is known regarding the molecular organization of the young reticulocyte membrane and skeletal network. This proposal is designed to apply various techniques, including protein electrophoresis, Western blot analysis. immunotluorescence microscopy, cell culture, and single cell micropipette manipulation, to investigate the molecular organization and dynamics of membrane and skeletal molecules in the young reticulocyte and the effect of specific liganding on membrane organization and mechanical properties of the skeletal network.

在红细胞生成结束时，成红细胞排出它们的细胞核， 网状细胞在这个过程中，质膜成分的分配 挤压核和年轻的网织红细胞之间的关系是由 膜和骨架网络的分子组织。的差异 网织红细胞和红细胞的形态和机械特性表明 网织红细胞成熟过程中膜组织的改变。等 组织结构的变化也可能导致粘附功能的丧失 分子，如Lu，LW和CD47。很少有人知道关于 年轻网织红细胞膜和骨骼肌的分子结构 网络本提案旨在应用各种技术，包括 蛋白质电泳、Western印迹分析。免疫荧光显微术， 细胞培养和单细胞微量移液器操作，以研究 膜和骨架分子的分子组织和动力学 年轻网织红细胞及其膜特异性配体的作用 组织和机械性能的骨架网络。