Ultrasonic Method for Testing Anti Sickling Agents

超声波法测试抗镰状化剂

• 批准号: 6527580
• 负责人: MARTIN Carl BARUCH
• 金额: $ 34.85万
• 依托单位: EMPIRICAL TECHNOLOGIES CORPORATION
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6527580
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; diagnostic tests; drug screening /evaluation; hemoglobin Ss; human subject; patient oriented research; sickle cell anemia; sickling inhibitor; technology /technique development; viscosity

DESCRIPTION (provided by applicant): The results of the Phase I effort, during the course of which the blood samples of 7 HbSS patients were examined, established the feasibility of using phase-sensitive acoustic detection techniques to quantitatively monitor the polymerization of HbSS. The effects of introducing anti-sickling agents were also clearly resolved in the results. In addition, the acoustic measurements performed on 5 normal blood samples revealed the techniques sensitivity by demonstrating the reproducible resolution of the minute acoustic response to de-oxygenation of normal blood. With the basic response question settled, the primary aim of the Phase II effort will be to develop a laboratory prototype that can be used by a third party to support anti-sickling drug research efforts, general sickle cell research, as well as be potentially used as a potential diagnostic tool in clinical settings. In order to accomplish this goal, the following specific aims will be pursued under Phase II: 1. An improved experimental prototype will be designed. 2. A stable and repeatable test substrate (cell suspension) will be developed. 3. The device will be characterized extensively with regard to calibration, sensitivity, and sample variability. 4. The effects of an extensive number of anti-sickling agents will be measured. PROPOSED COMMERCIAL APPLICATION: Not Available

描述（由申请人提供）：第一阶段努力的结果， 检查了7个HBSS患者的血液样本的过程， 建立了使用相敏感检测的可行性 定量监测HBSS聚合的技术。效果 在结果中也清楚地解决了引入反踢剂。在 此外，对5个正常血液样本进行的声学测量 通过证明可重复的 分钟对正常血液脱氧的声学反应的分辨率。 随着基本回答问题的解决，第二阶段的主要目的 努力是开发一个实验室原型，该原型可以由三分之一使用 支持反访问药物研究工作的政党，一般镰状细胞 研究，并有可能用作潜在的诊断工具 临床环境。为了实现这一目标，以下具体 目标将在II阶段：1中追求。改进的实验原型将 设计。 2。稳定且可重复的测试基材（细胞悬浮液）将 被开发。 3。该设备将被广泛表征 校准，灵敏度和样本变异性。 4。 将测量大量的反踢剂。 拟议的商业应用： 无法使用