Developing a Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS) Platform to Study the Structure and Assembly of Bacterial Nanocompartments.

开发氢/氘交换质谱 (HDX-MS) 平台来研究细菌纳米室的结构和组装。

• 批准号: 1940602
• 金额: --
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1940602
• 关键词: Developing; Hydrogen; Deuterium; Exchange; Mass

Bacterial nanocompartments (BNCs) are large protein assemblies comprising a hollow icosahedral multimeric protein cage which can sequester a range of 'cargo' protein (often enzymes) inside. This project will develop techniques in hydrogen/deuterium exchange mass spectrometry (HDX-MS) to study the structure, dynamics and assembly of BNCs. These mass spectrometry studies will complement existing structural biology studies using X-ray crystallography and electron microscopy. Understanding these fascinating systems will allow their future manipulation as potential platforms for biotechnology, synthetic biology, and drug delivery. The student will work in a highly interdisciplinary team spanning the disciplines of chemistry, biology and engineering. The student will learn skills in protein mass spectrometry, recombinant protein production, biochemistry, and structural biology. Protein crystallography and electron microscopy will be performed in collaboration with Dr. Jon Marles-Wright, University of Newcastle (www.marles-wright-lab.org).

细菌纳米区室（BNC）是包含中空二十面体多聚体蛋白质笼的大蛋白质组装体，所述中空二十面体多聚体蛋白质笼可以将一系列“货物”蛋白质（通常是酶）隔离在内部。该项目将开发氢/氘交换质谱（HDX-MS）技术，以研究BNC的结构，动力学和组装。这些质谱研究将补充现有的结构生物学研究，使用X射线晶体学和电子显微镜。了解这些迷人的系统将允许他们未来的操纵作为生物技术，合成生物学和药物输送的潜在平台。学生将在一个高度跨学科的团队中工作，涵盖化学，生物学和工程学等学科。学生将学习蛋白质质谱，重组蛋白生产，生物化学和结构生物学的技能。蛋白质晶体学和电子显微镜将与纽卡斯尔大学的Jon Marles-Wright博士（www.marles-wright-lab.org）合作进行。

项目成果

Mass spectrometry reveals the assembly pathway of encapsulated ferritins and highlights a dynamic ferroxidase interface.

质谱分析揭示了封装铁蛋白的组装途径，并突出了动态亚铁氧化酶界面。

• DOI: 10.1039/c9cc08130e
• 发表时间: 2020
• 期刊: Chemical communications (Cambridge, England)
• 作者: Ross J

Teaching Chemistry in Higher Education: A Festschrift in Honour of Professor Tina Overton

高等教育化学教学：纪念蒂娜·奥弗顿教授的纪念文集

• 发表时间: 2019
• 作者: Michael Seery, M. K., Seery