Instrumentation For Spectral Karyotyping

光谱核型分析仪器

• 批准号: 6548625
• 负责人: Paul D Smith
• 金额: --
• 依托单位: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6548625
• 关键词: biomedical equipment development; chromosome deletion; chromosome inversion; chromosome translocation; interferometry; karyotype

Spectral karyotyping is an evolving cytogenetic technology to identify chromosomal rearrangements such as deletions, inversions, and translocations seen in cancer cell lines. A commercial instrument operates as a modified Sagnac interferometer installed on an epi-illumination microscope to capture the spectral signature of probes used to paint chromosomes. The epi-illumination port and the fluorescent cube assembly of the microscope have been modified to permit single wavelength excitation, resulting in greater than an order of magnitude improvement in the signal to noise ratio of the fluorescent signals and a reduction in the overall background signal. These improvements will permit resolution of smaller chromosome band abnormalities and identification of their correlation with disease.

光谱核型分析是一种不断发展的细胞遗传学技术，用于鉴定癌细胞系中出现的染色体重排，如缺失、倒位和易位。一种商业仪器作为安装在落射照明显微镜上的修改后的Sagnac干涉仪来捕获用于绘制染色体的探针的光谱特征。落射照明端口和荧光立方体组件的显微镜已被修改，允许单波长激发，导致大于一个数量级的改善的信号噪声比的荧光信号和减少的整体背景信号。这些改进将允许分辨率较小的染色体带异常和鉴定其与疾病的相关性。